Antifungal activity of chitosan-silver nanoparticle composite against Colletotrichum gloeosporioides associated with mango anthracnose
Antifungal activity of chitosan-silver nanoparticle composite against Colletotrichum gloeosporioides associated with mango anthracnose
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壳聚糖-银纳米粒子复合物对芒果炭疽病炭疽病的抗真菌活性
DOI：
10.5897/ajmr2013.6584
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发表时间：
2014
影响因子：
--
通讯作者：
S. Madhura
中科院分区：
文献类型：
--
作者：
P. Chowdappa;Shivakumar Gowda;C. Chethana;S. Madhura
负载有各种金属离子如Ag+、Cu 2+、Zn 2+、Mn 2+和Fe 2+的壳聚糖已被报道发挥强的抗微生物活性。在这项研究中，在95°C下使用壳聚糖作为还原剂和稳定剂合成了银纳米颗粒（AgNPs）。紫外-可见光谱显示在415-420 nm之间的范围内的峰，这是银纳米颗粒的特征表面等离子体共振带。使用场发射扫描电子显微镜结合能量色散X射线光谱法研究所得纳米粒子的尺寸、形状和聚集特性。结果表明，壳聚糖-银纳米粒子（chitosan-AgNP）复合物表面光滑，平均流体动力学直径为495-616 nm，银纳米粒子的粒径分布为10 ~ 15 nm。壳聚糖-AgNP复合物的zeta电位为+50.08 mV至+87.75 mV。离体孢子萌发试验表明，壳聚糖-银纳米粒子复合物对炭疽菌的抑菌活性显著高于其组分。使用离体芒果果实的体内测定。Alphonso实验表明壳聚糖-AgNP复合物对炭疽病有显著的抑制作用。因此，这项研究表明，芒果采后腐烂可以最大限度地减少壳聚糖-银纳米复合材料，其在商业规模上的应用需要加以利用。
关键词：壳聚糖，银纳米粒子，抗真菌活性，芒果果实，炭疽菌。
Chitosan loaded with various metal ions such as Ag+, Cu2+, Zn2+, Mn2+ and Fe2+ has been reported to exert strong antimicrobial activity. In this study, the silver-nanoparticles (AgNPs) were synthesized at 95°C using chitosan as the reducing agent and stabilizer. The UV-Vis spectrum displayed peak in a range between 415-420 nm, the characteristic surface plasmon resonance band of silver nanoparticles. The size, shape and aggregation properties of the resultant nanoparticles were examined using field emission scanning electron microscopy coupled with energy dispersive X-ray spectroscopy. The measurement results indicated that the chitosan-silver nanoparticle (chitosan-AgNP) composite having the mean hydrodynamic diameter range of 495-616 nm were apparently smooth and the silver nanoparticles with the size distribution was from 10 to 15 nm. Chitosan-AgNP composites had a zeta potential of +50.08 mV to +87.75 mV. In vitro conidial germination assay indicated that chitosan-AgNP composite exhibited significantly higher antifungal activity against Colletotrichum gloeosporioides than its components at their respective concentrations. In vivo assay using detached mango fruit cv. Alphonso showed that anthracnose was significantly inhibited by chitosan-AgNP composite. Therefore, this study suggests that postharvest decay in mango can be minimized by chitosan-AgNP composites and its application on a commercial scale needs to be exploited.
Key words: Chitosan, silver nanoparticle, antifungal activity, mango fruits, Colletotrichum gloeosporioides.