Characteristic tetraspanin expression patterns mark various tissues during early Xenopus development
Characteristic tetraspanin expression patterns mark various tissues during early Xenopus development
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DOI：
10.1111/dgd.12836
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发表时间：
2023-01-24
影响因子：
2.5
通讯作者：
Tanaka，Masamitsu
中科院分区：
文献类型：
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作者：
Kuriyama，Sei;Tanaka，Masamitsu
四跨膜蛋白（tetraspanins，Tspan）是一类具有四个跨膜结构域的细胞表面蛋白。Tspan存在于细胞质膜和各种细胞器的外泌体上。关于Tspans在Xenopus早期发育中的功能的报道主要集中在配子体中uroplakins的表达。尽管细胞外囊泡（EV）（包括外来体）的作用已在癌症研究中得到积极分析，但EV对早期发育的贡献尚未得到很好的理解。这是因为电动汽车的扩散性与非常严格的发展过程不相容。在这项研究中，我们分析了非洲爪蟾早期发育中的Tspan家族成员。在特定器官如脊索、眼、颅神经嵴细胞（CNCs）、躯干神经嵴细胞、基板和体节中表达显著。我们在体外和体内的CNCs中过表达几种Tspan组合。改变伴侣改变了荧光标记的Tspan的分布。因此，它表明，在一个特定的组织中的多个Tspan的表达可能会产生异质性的细胞间通讯，这还没有被认识到。
The tetraspanins (Tspans) constitute a family of cell surface proteins with four transmembrane domains. Tspans have been found on the plasma membrane and on exosomes of various organelles. Reports on the function of Tspans during the early development ofXenopushave mainly focused on the expression of uroplakins in gametes. Although the roles of extracellular vesicles (EVs) including exosomes have been actively analyzed in cancer research, the contribution of EVs to early development is not well understood. This is because the diffusivity of EVs is not compatible with a very strict developmental process. In this study, we analyzed members of the Tspan family in early development ofXenopus. Expression was prominent in specific organs such as the notochord, eye, cranial neural crest cells (CNCs), trunk neural crest cells, placodes, and somites. We overexpressed several combinations of Tspans in CNCs in vitro and in vivo. Changing the partner changed the distribution of fluorescent‐labeled Tspans. Therefore, it is suggested that expression of multiple Tspans in a particular tissue might produce heterogeneity of intercellular communication, which has not yet been recognized.