Genomic characterization of an emerging Enterobacteriaceae species: the first case of co-infection with a typical pathogen in a human patient
Genomic characterization of an emerging Enterobacteriaceae species: the first case of co-infection with a typical pathogen in a human patient
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新兴肠杆菌科物种的基因组特征：首例人类患者与典型病原体共同感染的病例
DOI：
10.1186/s12864-020-6720-z
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发表时间：
2020-03
期刊：
影响因子：
4.4
通讯作者：
Gao Zhancheng
中科院分区：
文献类型：
--
作者：
Zhang Zhao;Li Daixi;Shi Xing;Zhai Yao;Guo Yatao;Zheng Yali;Zhao Lili;He Yukun;Chen Yusheng;Wang Zhanwei;Su Jianrong;Kang Yu;Gao Zhancheng
背景病原菌常引起耐药菌感染，对临床实践具有重要意义。然而，很少有记录的许多新兴的机会致病菌及其生物学特性。在此，我们从一位胆道感染的病人身上分离出一株产超广谱β-内酰胺酶的肠杆菌。我们探讨了该菌株的生物学和基因组特征，以提供新的证据和详细的信息，机会致病菌的共同感染，他们可能cause.ResultsThe分离生长非常缓慢，但赋予强大的保护共同感染头孢菌素敏感克雷伯氏肺炎。由于最初的实验室检测未能确定菌株的分类，给患者的病原学诊断和抗感染治疗带来了极大的困惑。严格的测序工作获得了我们命名为AF 18的分离株的完整基因组序列。AF 18与从土壤、临床污水和患者中分离的少数菌株在遗传学上接近，共同形成一个新的种，但其分类命名仍在讨论中。这是人类感染这种新物种的第一份报告。与其近缘种一样，AF 18携带许多与细胞运动性相关的基因、多种适应自然环境和动物宿主的基因、超过30个移动的遗传元件和携带blaCTX-M-3基因的质粒，表明其能够从自然环境向患者传播抗生素耐药基因。转录组测序确定了两个sRNAs，关键调节AF 18的生长速度，这可能是作为新的抗菌strategies.ConclusionsOur研究结果意味着，AF 18及其物种不仅感染相关的，但也是潜在的传播者的抗生素耐药基因，这突出了需要持续监测这种新的物种和努力制定治疗策略。
BackgroundOpportunistic pathogens are important for clinical practice as they often cause antibiotic-resistant infections. However, little is documented for many emerging opportunistic pathogens and their biological characteristics. Here, we isolated a strain of extended-spectrum β-lactamase-producingEnterobacteriaceaefrom a patient with a biliary tract infection. We explored the biological and genomic characteristics of this strain to provide new evidence and detailed information for opportunistic pathogens about the co-infection they may cause.ResultsThe isolate grew very slowly but conferred strong protection for the co-infected cephalosporin-sensitiveKlebsiella pneumoniae. As the initial laboratory testing failed to identify the taxonomy of the strain, great perplexity was caused in the etiological diagnosis and anti-infection treatment for the patient. Rigorous sequencing efforts achieved the complete genome sequence of the isolate which we designated as AF18. AF18 is phylogenetically close to a few strains isolated from soil, clinical sewage, and patients, forming a novel species together, while the taxonomic nomenclature of which is still under discussion. And this is the first report of human infection of this novel species. Like its relatives, AF18 harbors many genes related to cell mobility, various genes adaptive to both the natural environment and animal host, over 30 mobile genetic elements, and a plasmid bearingblaCTX-M-3gene, indicating its ability to disseminate antimicrobial-resistant genes from the natural environment to patients. Transcriptome sequencing identified two sRNAs that critically regulate the growth rate of AF18, which could serve as targets for novel antimicrobial strategies.ConclusionsOur findings imply that AF18 and its species are not only infection-relevant but also potential disseminators of antibiotic resistance genes, which highlights the need for continuous monitoring for this novel species and efforts to develop treatment strategies.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
3.2
作者：
Hung-Yi Wu;P. Chung;Hsiao-Wei Shih;Sy-Ray Wen;E. Lai
通讯作者：
Hung-Yi Wu;P. Chung;Hsiao-Wei Shih;Sy-Ray Wen;E. Lai
影响因子：
5.8
作者：
Gardner, Shea N.;Slezak, Tom;Hall, Barry G.
通讯作者：
Hall, Barry G.
DOI：
10.1128/genomea.01432-16
发表时间：
2016-12-22
期刊：
Genome announcements
影响因子：
--
作者：
Tetz G;Tetz V
通讯作者：
Tetz V
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P