SEROLOGICAL RESPONSES IN CHIMPANZEES INOCULATED WITH HUMAN-IMMUNODEFICIENCY-VIRUS GLYCOPROTEIN (GP120) SUBUNIT VACCINE
SEROLOGICAL RESPONSES IN CHIMPANZEES INOCULATED WITH HUMAN-IMMUNODEFICIENCY-VIRUS GLYCOPROTEIN (GP120) SUBUNIT VACCINE
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DOI：
10.1073/pnas.84.23.8583
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发表时间：
1987-12-01
影响因子：
11.1
通讯作者：
FISCHINGER, PJ
中科院分区：
文献类型：
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作者：
ARTHUR, LO;PYLE, SW;FISCHINGER, PJ
一种人类免疫缺陷病毒(HIV)的主要包膜糖蛋白已被提纯，并被用于黑猩猩的原型疫苗。从感染人T淋巴细胞病毒(HTLV)-IIIB的细胞膜上纯化了12万道尔顿糖蛋白(GP 120)，最终制备的制剂含有低水平的或根本检测不到HTLV-IIIB核心抗原(P24)和低水平的内毒素。接种gp120的黑猩猩产生抗体，沉淀放射性标记的gp120，并在体外中和HTLV-IIIB的感染。在gp120免疫的黑猩猩体内未检测到HTLV-IIIB p24抗体。免疫动物的外周血白细胞检测T4+和T8+细胞，没有发现T4/T8比值下降，这表明用与T4结合的配体(Gp120)免疫对T4+细胞的数量没有明显的不良影响。目前唯一可以重复感染艾滋病毒的动物模型是黑猩猩。用HIV蛋白免疫黑猩猩将提供一个实验系统，用于测试原型疫苗在体内预防HIV感染的有效性。
The major envelope glycoprotein of a human immunodeficiency virus (HIV) has been purified and was utilized as a prototype vaccine in chimpanzees. The 120,000-dalton glycoprotein (gp 120) was purified from membranes of human T-lymphotropic virus (HTLV)-IIIB-infected cells and the final preparation contained low levels to no detectable HTLV-IIIB core antigen (p24) and low levels of endotoxin. Chimpanzees inoculated with gp120 responded by developing antibodies that precipitated radiolabeled gp120 and neutralized in vitro infection of HTLV-IIIB. Antibodies to HTLV-IIIB p24 were not detected in the gp120-immunized chimpanzees. Peripheral blood leukocytes from the vaccinated animals were examined for T4+ and T8+ cells, and no decrease in the T4/T8 ratio was found, indicating that immunization with a ligand (gp120) that binds to T4 has no detectable adverse effect on the population of T4+ cells. The only current animal model that can be reproducibly infected with HIV is the chimpanzee. Immunization of chimpanzees with HIV proteins will provide an experimental system for testing the effectiveness of prototype vaccines for preventing HIV infection in vivo.