DOUBLE-TILT ELECTRON TOMOGRAPHY
DOUBLE-TILT ELECTRON TOMOGRAPHY
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DOI：
10.1016/0304-3991(95)00078-x
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发表时间：
1995-10-01
期刊：
影响因子：
2.2
通讯作者：
FRANK, J
中科院分区：
文献类型：
--
作者：
PENCZEK, P;MARKO, M;FRANK, J
通过组合围绕两个正交轴的倾斜序列，在不增加投影数目的情况下，提高了层析重建的保真度，并且减少了重建伪影。用高压电磁场对大鼠肝线粒体进行实验重建，制作了0.6微米厚的塑料切片。比较了几种选择投影倾角的方案。描述了一种对齐基准标记的新方法。它使用迭代算法来确定每个倾斜图像的平移、比例、平面内旋转和倾角，强制执行基准标记在3D空间中的预期位置的一致。这些3D位置被用来找出两个倾斜序列与两组投影之间的方向。
Fidelity of tomographic reconstructions is improved and reconstruction artifacts are reduced, without increasing the number of projections, by combining tilt series taken around two orthogonal axes. Test reconstructions were made from high-voltage EM of rat liver mitochondria in a 0.6 mu m thick plastic section. A number of schemes for selecting tilt angles for the projections are compared. A new method for aligning fiducial markers is described. It uses an iterative algorithm to determine the shift, scale, in-plane rotation and tilt angle for each tilt image, enforcing agreement of the expected locations of the fiducial markers in 3D space. These 3D locations are used to find the orientation between two tilt series acid to both sets of projections.