Interaction of PAR-6 with CDC-42 is required for maintenance but not establishment of PAR asymmetry in C-elegans
Interaction of PAR-6 with CDC-42 is required for maintenance but not establishment of PAR asymmetry in C-elegans
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DOI：
10.1016/j.ydbio.2006.08.002
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发表时间：
2006-11-15
影响因子：
2.7
通讯作者：
Kemphues, Kenneth J.
中科院分区：
文献类型：
--
作者：
Aceto, Donato;Beers, Melissa;Kemphues, Kenneth J.
秀丽隐杆线虫胚胎极性需要不对称分布的蛋白质PAR-3、PAR-6和PKC-3。rho家族的GTCRs CDC-42调节这些蛋白质在哺乳动物、苍蝇和蠕虫中的活性。阐明其在C.我们在体内破坏PAR-6和CDC-42之间的相互作用，并且还确定了GFP标记的CDC-42在早期胚胎中的分布。突变PAR-6蛋白不能与CDC-42相互作用的积累不对称，在一个降低的水平，但这种不对称性并没有保持在第一次分裂。我们还确定了组成型活性GFP：：CDC-42在第一个细胞周期期间在与PAR-6重叠的结构域中富集在前部。这种不对称性依赖于PAR-2、PAR-5和PAR-6。此外，我们发现组成型活性GFP：：CDC-42的过表达增加了前结构域的大小。我们的结论是CDC-42与PAR-6的相互作用是不需要的初始建立的不对称性，但需要最大皮质积累的PAR-6，并保持其不对称性。(c)2006年爱思唯尔公司All rights reserved.
Caenorhabditis elegans embryonic polarity requires the asymmetrically distributed proteins PAR-3, PAR-6 and PKC-3. The rho family GTPase CDC-42 regulates the activities of these proteins in mammals, flies and worms. To clarify its mode of action in C. elegans we disrupted the interaction between PAR-6 and CDC-42 in vivo, and also determined the distribution of GFP-tagged CDC-42 in the early embryo. Mutant PAR-6 proteins unable to interact with CDC-42 accumulated asymmetrically, at a reduced level, but this asymmetry was not maintained during the first division. We also determined that constitutively active GFP: :CDC-42 becomes enriched in the anterior during the first cell cycle in a domain that overlaps with PAR-6. The asymmetry is dependent on PAR-2, PAR-5 and PAR-6. Furthermore, we found that overexpression of constitutively active GFP: :CDC-42 increased the size of the anterior domain. We conclude that the CDC-42 interaction with PAR-6 is not required for the initial establishment of asymmetry but is required for maximal cortical accumulation of PAR-6 and to maintain its asymmetry. (c) 2006 Elsevier Inc. All rights reserved.