Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography
Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography
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DOI：
10.1007/bf02975184
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发表时间：
2001-10-01
影响因子：
6.7
通讯作者：
Kim, YH
中科院分区：
文献类型：
--
作者：
Kang, JS;Linh, PT;Kim, YH
采用反相高效液相色谱法测定了韩国不同刺五加中刺五加苷B和E的含量。固定相为Zorbax 300 SB C-18，流动相为6%乙腈，流动2 min，以1.0 ml/min的流速，线性梯度至17%乙腈，流动18 min。在紫外210 nm下监测柱出水。将样品中刺五苦苷B和E对应的各峰的保留时间和LC/MS谱与标准品相比较进行鉴定。总体上，刺叶皂苷B和E在茎中的含量高于根中的含量。根据刺五加苷B和E的含量可将刺五加分为两类：一类不含或极少量刺五加苷B，另一类既含刺五加苷B又含刺五加苷E。
Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected in Korea. The stationary phase used was Zorbax 300 SB C-18 and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and E in stems were higher than those in roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.