Species-specific physiological response of dinoflagellates to quantified small-scale turbulence
Species-specific physiological response of dinoflagellates to quantified small-scale turbulence
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DOI：
10.1111/j.1529-8817.2007.00392.x
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发表时间：
2007-10-01
影响因子：
2.9
通讯作者：
Estrada, Marta
中科院分区：
文献类型：
--
作者：
Berdalet, Elisa;Peters, Francesc;Estrada, Marta
湍流已被证明可以改变一些甲藻生理学的不同方面。反应似乎是物种特异性的，并依赖于用于产生小规模湍流的实验设计和设置。采用Berdalet（1992）对赤藻（Akashiwo sanguarum（Hirasaka）Ge）的实验设计，研究了3种甲藻对湍流响应的变异性。汉森等Moestrup（Gymnastinium nelsonii G.）W. Martin）。在所有实验中，湍流是由轨道振荡器以100 rpm的速度产生的，这对应于整体平均值，耗散率（λ，使用声学多普勒测速仪量化）约为2 cm（2）。s（-3）。湍流没有明显影响裸藻，一种小型的甲藻然而，微小亚历山大藻Halim和Triestinum J. Schiller在指数生长期振荡时表现出降低的净生长率（分别为33%和28%）。与静置培养相比，A. minutum和P. triestinum使平均细胞体积（分别高达1.4和2.5倍）和平均DNA含量（分别高达1.8和5.3倍）增加。受湍流影响的培养物在返回静止条件时恢复其正常细胞特性。暴露于搅动的细胞的游泳速度是未摇动的细胞的一半。总的来说，A. minutum和P. triestinum的荧光强度与以前在A.血腥我们没有发现与分类学或形态学相关的明显趋势。
Turbulence has been shown to alter different aspects of the physiology of some dinoflagellates. The response appears to be species-specific and dependent on the experimental design and setup used to generate small-scale turbulence. We examined the variability of the response of three dinoflagellate species to the turbulence, following the same experimental design used by Berdalet (1992) on Akashiwo sanguinea (Hirasaka) Ge. Hansen et Moestrup (=Gymnodinium nelsonii G. W. Martin). In all experiments, turbulence was generated by an orbital shaker at 100 rpm, which corresponded on bulk average, to dissipation rates (epsilon, quantified using an acoustic Doppler velocimeter) of approximate to 2 cm(2) . s(-3). Turbulence did not appreciably affect Gymnodinium sp., a small dinoflagellate. However, Alexandrium minutum Halim and Prorocentrum triestinum J. Schiller exhibited a reduced net growth rate (33% and 28%, respectively) when shaken during the exponential growth phase. Compared to the still cultures, the shaken treatments of A. minutum and P. triestinum increased the mean cell volume (up to 1.4- and 2.5-fold, respectively) and the mean DNA content (up to 1.8- and 5.3-fold, respectively). Cultures affected by turbulence recovered their normal cell properties when returned to still conditions. The swimming speed of the cells exposed to agitation was half that of the unshaken ones. Overall, the response of A. minutum and P. triestinum was similar, but with lower intensity, to that observed previously on A. sanguinea. We found no clear trends related to taxonomy or morphology.