Cation fluxes and (Na+ + K+)-activated ATPase activity in erythrocytes of patients with essential hypertension.
Cation fluxes and (Na+ + K+)-activated ATPase activity in erythrocytes of patients with essential hypertension.
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原发性高血压患者红细胞中的阳离子通量和 (Na K ) 激活的 ATP 酶活性。
DOI：
10.3109/10641968109033706
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发表时间：
1981
影响因子：
12.3
通讯作者：
A. van't Laar
中科院分区：
文献类型：
--
作者：
H. Swarts;S. L. Bonting;J. De Pont;F. Schuurmans Stekhoven;T. Thien;A. van't Laar
近年来，对于原发性高血压患者的阳离子泵和红细胞通量异常提出了各种不同的说法，但部分是相互矛盾的。鉴于此类异常对于诊断目的的明显意义，并且可能对于我们了解原发性高血压的病理生理学而言，我们对这些说法进行了研究。我们测定了原发性高血压患者和血压正常患者的红细胞的以下参数： 1. (Na+ + K+)-ATPase 活性以及 Na+、K+ 和 ATP 的 Km 值； 2.冷处理后和用对氯汞苯磺酸盐处理后富Na+细胞中哇巴因敏感的Na+和K+通量； 3. Na+ + K+ 的速尿敏感、哇巴因不敏感的共转运流出。除了高血压患者冷治疗后哇巴因敏感性 K+ 流入略有减少外，没有观察到显着差异。因此，我们得出结论，红细胞中这些参数的测定似乎对原发性高血压的诊断没有用。
Various claims, partially conflicting, have been made in recent years for abnormalities in cation pumps and fluxes in erythrocytes of patients with essential hypertension. In view of the obvious significance of such abnormalities for diagnostic purposes, and possibly for our understanding of the pathophysiology of essential hypertension, we have investigated these claims. We have determined the following parameters of erythrocytes from essential hypertensives and normotensives: 1. (Na+ + K+)-ATPase activity and the Km values for Na+, K+ and ATP; 2. ouabain-sensitive fluxes of Na+ and K+ in Na+-enriched cells after cold treatment and after treatment with p-chloromercuribenzenesulphonate; 3. furosemide-sensitive, ouabain-insensitive cotransport efflux of Na+ + K+. No significant differences were observed, except for a slight decrease in the ouabain-sensitive K+ influx after cold treatment in hypertensives. Hence, we conclude that determination of these parameters in erythrocytes does not seem to be useful for the diagnosis of essential hypertension.