Cationic Conjugated Polymers-Induced Quorum Sensing of Bacteria Cells
Cationic Conjugated Polymers-Induced Quorum Sensing of Bacteria Cells
复制标题
阳离子共轭聚合物诱导细菌细胞的群体感应
DOI：
10.1021/acs.analchem.5b03920
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发表时间：
2016
影响因子：
7.4
通讯作者：
Wang Shu
中科院分区：
文献类型：
--
作者：
Zhang Pengbo;Lu Huan;Chen Hui;Zhang Jiangyan;Liu Libing;Lv Fengting;Wang Shu
Bacteria quorum sensing (QS) has attracted significant interest for understanding cell–cell communication and regulating biological functions. In this work, we demonstrate that water-soluble cationic conjugated polymers (PFP-G2) can interact with bacteria to form aggregates through electrostatic interactions. With bacteria coated in the aggregate, PFP-G2 can induce the bacteria QS system and prolong the time duration of QS signal molecules (autoinducer-2 (AI-2)) production. The prolonged AI-2 can bind with specific protein and continuously regulate downstream gene expression. Consequently, the bacteria show a higher survival rate against antibiotics, resulting in decreased antimicrobial susceptibility. Also, AI-2 induced by PFP-G2 can stimulate 55.54 ± 12.03% more biofilm inE. coli. This method can be used to understand cell–cell communication and regulate biological functions, such as the production of signaling molecules, antibiotics, other microbial metabolites, and even virulence.
