LncRNA NONHSAT030515 promotes the chondrogenic differentiation of human adipose-derived stem cells via regulating the miR-490-5p/BMPR2 axis.
LncRNA NONHSAT030515 promotes the chondrogenic differentiation of human adipose-derived stem cells via regulating the miR-490-5p/BMPR2 axis.
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DOI：
10.1186/s13018-021-02757-z
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发表时间：
2021-11-06
影响因子：
2.6
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Yang Q;Guo J;Ren Z;Li B;Huang H;Yang Z
人脂肪源性干细胞（hADSCs）的成软骨分化对软骨的生成和降解至关重要。lncrna在干细胞分化过程中发挥着重要作用。然而，lncRNA在hscs中的作用和机制尚不清楚。我们之前的研究表明，miR-490-5p在hscs的软骨分化过程中下调。在本研究中，我们研究了lncRNA NONHSAT030515与miR-490-5p相互作用对hscs软骨分化的影响和机制。阿利新蓝染色评估人脂肪干细胞软骨分化后软骨基质蛋白的沉积。免疫组化法检测胶原ii的表达。应用TargetScan、miRTarBase和miRDB数据库分析lncRNA NONHSAT030515的miRNA和靶基因。通过双荧光素酶实验确定NONHSAT030515的直接靶点。通过转染pcDNA3.1- NONHSAT030515和sh- NONHSAT030515，验证lncRNA NONHSAT030515在软骨分化中的作用。采用qRT-PCR和Western blot检测Aggrecan、SOX9和COL2A1的表达水平。阿利新蓝染色、免疫细胞化学、qRT-PCR、Western blot均证实lncRNA NONHSAT030515可促进hscs软骨分化。MiR-490- 5p是NONHSAT030515的直接靶基因，而BMPR2是靶基因。荧光素酶报告基因实验证实了这一结果。上调NONHSAT030515可促进BMPR2蛋白表达，促进软骨细胞分化，而下调NONHSAT030515则完全相反。LncRNA NONHSAT030515通过调节miR-490- 5p增加BMPR2的表达，促进hADSCs的软骨分化。
Chondrogenic differentiation of human adipose-derived stem cells (hADSCs) is important for cartilage generation and degradation. LncRNAs play an essential role in stem cell differentiation. However, the role and mechanism of lncRNA in hADSCs remain unclear. Our previous study showed that miR-490-5p was downregulated during chondrogenic differentiation of hADSCs. In this study, we investigated the effect and mechanism of lncRNA NONHSAT030515 interacting with miR-490-5p on chondrogenic differentiation of hADSCs. Alcian blue staining was used to assess the deposition of chondromatrix proteins following chondrogenic differentiation of human adipose stem cells. Immunohistochemistry was used to evaluate the expression of collagenII. TargetScan, miRTarBase and miRDB database analyses were applied to find the miRNA and target genes of lncRNA NONHSAT030515. A dual luciferase experiment was conducted to identify the direct target of NONHSAT030515. pcDNA3.1- NONHSAT030515 transfection and sh- NONHSAT030515 treatment were conducted to verify the role of lncRNA NONHSAT030515 in chondrogenic differentiation. The levels of Aggrecan, SOX9 and COL2A1 were analyzed by qRT-PCR and Western blot assay. Alcian blue staining, immunocytochemical, qRT-PCR, and Western blot have determined that lncRNA NONHSAT030515 can promote the chondrogenic differentiation of hADSCs. MiR-490- 5p was the direct target of NONHSAT030515, while BMPR2 was the target gene. This result was confirmed by luciferase reporter assay. Up-regulation of NONHSAT030515 promoted BMPR2 protein expression and promoted chondrogenic differentiation, whereas down-regulation of NONHSAT030515 caused completely opposite results. LncRNA NONHSAT030515 promotes the chondrogenic differentiation of hADSCs through increasing BMPR2 expression by regulating miR-490- 5p.
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影响因子：
3.7
作者：
Liao JY;Ma LM;Guo YH;Zhang YC;Zhou H;Shao P;Chen YQ;Qu LH
通讯作者：
Qu LH
DOI：
10.1242/dev.152504
发表时间：
2017-12-15
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Barter MJ;Gomez R;Hyatt S;Cheung K;Skelton AJ;Xu Y;Clark IM;Young DA
通讯作者：
Young DA
LncRNA SNHG5 调控的 miR-23a-3p 通过靶向 SOX6/SOX5 抑制人脂肪干细胞的软骨分化
影响因子：
3.6
作者：
Yang, Zhen;Ren, Zhijing;Tian, Xiaobin
通讯作者：
Tian, Xiaobin
CERNA假设：隐藏RNA语言的Rosetta石头？
影响因子：
64.5
作者：
Salmena L;Poliseno L;Tay Y;Kats L;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
4.6
作者：
Steinbusch MMF;Caron MMJ;Surtel DAM;Friedrich F;Lausch E;Pruijn GJM;Verhesen W;Schroen BLM;van Rhijn LW;Zabel B;Welting TJM
通讯作者：
Welting TJM