Duration of humoral immunity to common viral and vaccine antigens
Duration of humoral immunity to common viral and vaccine antigens
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DOI：
10.1056/nejmoa066092
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发表时间：
2007-11-08
影响因子：
158.5
通讯作者：
Slifka, Mark K.
中科院分区：
文献类型：
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作者：
Amanna, Ian J.;Carlson, Nichole E.;Slifka, Mark K.
背景维持长期抗体反应对于针对许多病原体的保护性免疫至关重要。然而，体液免疫的持续时间和记忆 B 细胞所发挥的作用仍不清楚。方法我们对 45 名受试者的病毒抗原（牛痘、麻疹、腮腺炎、风疹、水痘带状疱疹病毒和 Epstein-Barr 病毒）和非复制抗原（破伤风和白喉）特异性抗体滴度进行了纵向分析，持续时间长达 26年。此外，我们通过有限稀释分析测量了抗原特异性记忆 B 细胞，并将记忆 B 细胞频率与其相应的血清抗体水平进行了比较。结果抗病毒抗体反应非常稳定，半衰期从水痘带状疱疹病毒的估计 50 年到麻疹和腮腺炎等其他病毒的 200 多年不等。针对破伤风和白喉抗原的抗体反应减弱得更快，估计半衰期分别为 11 年和 19 年。 B 细胞记忆是长期存在的，但外周记忆 B 细胞数量与所测试的八种抗原中的五种的抗体水平之间没有显着相关性。结论这些研究对在十多年的过程中纵向跟踪的受试者中的多种抗原的血清学记忆进行了定量分析。在多次暴露或重复接种疫苗的情况下，记忆 B 细胞数量与抗体滴度并不相关。这一发现表明，外周记忆 B 细胞和抗体分泌浆细胞可能代表独立调节的细胞群，并且可能在保护性免疫的维持中发挥不同的作用。
BackgroundMaintenance of long-term antibody responses is critical for protective immunity against many pathogens. However, the duration of humoral immunity and the role played by memory B cells remain poorly defined.MethodsWe performed a longitudinal analysis of antibody titers specific for viral antigens (vaccinia, measles, mumps, rubella, varicella-zoster virus, and Epstein-Barr virus) and nonreplicating antigens (tetanus and diphtheria) in 45 subjects for a period of up to 26 years. In addition, we measured antigen-specific memory B cells by means of limiting-dilution analysis, and we compared memory B-cell frequencies to their corresponding serum antibody levels.ResultsAntiviral antibody responses were remarkably stable, with half-lives ranging from an estimated 50 years for varicella-zoster virus to more than 200 years for other viruses such as measles and mumps. Antibody responses against tetanus and diphtheria antigens waned more quickly, with estimated half-lives of 11 years and 19 years, respectively. B-cell memory was long-lived, but there was no significant correlation between peripheral memory B-cell numbers and antibody levels for five of the eight antigens tested.ConclusionsThese studies provide quantitative analysis of serologic memory for multiple antigens in subjects followed longitudinally over the course of more than one decade. In cases in which multiple exposures or repeated vaccinations were common, memory B-cell numbers did not correlate with antibody titers. This finding suggests that peripheral memory B cells and antibody-secreting plasma cells may represent independently regulated cell populations and may play different roles in the maintenance of protective immunity.