Altered placental expression of kisspeptin and its receptor in pre-eclampsia.
Altered placental expression of kisspeptin and its receptor in pre-eclampsia.
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DOI：
10.1530/joe-12-0091
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发表时间：
2012-07
期刊：
影响因子：
--
通讯作者：
Williams PJ
中科院分区：
文献类型：
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作者：
Cartwright JE;Williams PJ
Kisspeptin，最初被鉴定为转移抑制素，在预防癌症转移方面很重要，最近被证明在怀孕中很重要。kisspeptin在妊娠中的作用包括调节胎盘形成过程中滋养细胞的侵袭和迁移。妊娠特异性疾病先兆子痫（PE）现在被认为是开始与滋养层浸润不足，因此，目前的研究开始在整个妊娠期间和PE中检测胎盘中kisspeptin（KISS 1）及其受体（KISS 1R）的表达。胎盘组织来自接受选择性手术终止早孕的女性（n=10）和正常妊娠足月剖腹产的女性（n=10）和PE（n=10）。进行石蜡包埋切片的免疫组织化学和蛋白质免疫印迹以评估蛋白质定位和表达。进行定量实时PCR以评估KISS 1和KISS 1R两者的mRNA表达。蛋白质和mRNA的表达被发现是镜像对方与KISS 1的表达被发现在PE中的减少与正常足月妊娠相比。有趣的是，KISS1R在mRNA和蛋白水平的表达被发现在PE与正常足月妊娠相比，增加。目前KISS 1R表达增加的研究结果可能代表了KISS 1在PE中的功能活性高于正常妊娠的机制。高水平的KISS 1R活性可能参与抑制滋养层细胞侵袭和血管生成，这与PE有关。
Kisspeptin, originally identified as metastatin, important in preventing cancer metastasis, has more recently been shown to be important in pregnancy. Roles indicated for kisspeptin in pregnancy include regulating trophoblast invasion and migration during placentation. The pregnancy-specific disorder pre-eclampsia (PE) is now accepted to begin with inadequate trophoblast invasion and the current study therefore sets out to characterise placental expression of both kisspeptin (KISS1) and its receptor (KISS1R) throughout pregnancy and in PE. Placental tissue was obtained from women undergoing elective surgical termination of early pregnancy (n=10) and from women following Caesarean section at term in normal pregnancy (n=10) and with PE (n=10). Immunohistochemistry of paraffin embedded sections and western immunoblotting were performed to assess protein localisation and expression. Quantitative real-time PCR was carried out to evaluate mRNA expression of both KISS1 and KISS1R. Protein and mRNA expression was found to mirror each other with KISS1 expression found to be reduced in PE compared with that in normal term pregnancy. Interestingly, KISS1R expression at both the mRNA and protein levels was found to be increased in PE compared with that in normal term pregnancy. The current findings of increased KISS1R expression may represent a mechanism by which functional activity of KISS1 is higher in PE than in normal pregnancy. Higher levels of activity of KISS1R may be involved in inhibition of trophoblast invasion and angiogenesis, which are associated with PE.