Controlling Polymersome Surface Topology at the Nanoscale by Membrane Confined Polymer/Polymer Phase Separation
Controlling Polymersome Surface Topology at the Nanoscale by Membrane Confined Polymer/Polymer Phase Separation
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DOI：
10.1021/nn102455z
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发表时间：
2011-03-01
期刊：
影响因子：
17.1
通讯作者：
Battaglia, Giuseppe
中科院分区：
文献类型：
--
作者：
LoPresti, Caterina;Massignani, Marzia;Battaglia, Giuseppe
大自然有精妙的能力在宏观和纳米尺度上设计特定的表面模式和拓扑结构，这些都与精确的功能有关。遵循仿生方法，我们设计了完全合成的纳米颗粒，能够自组织其表面进入受控区域。我们专注于聚合囊泡或“聚合体”；两亲嵌段共聚物在水中通过自组装形成的封闭膜。利用不同聚合物相分离的内在热力学倾向，我们以不同比例混合不同的囊泡形成嵌段共聚物，以获得具有不同表面结构域的大范围聚合物体。采用激光共聚焦组合。扫描显微镜对微米大小的聚合体的研究，以及电子显微镜、原子力显微镜和荧光光谱对纳米大小的聚合体的研究，我们发现这些结构域在微观和纳米尺度上都表现出相似的形状，其尺寸与囊泡直径成线性比例。最后，我们证明了这种对这些聚合体表面“斑块性”的控制决定了它们对活细胞的细胞内化动力学。
Nature has the exquisite ability to design specific surface patterns and topologies on both the macro- and nanolength scales that relate to precise functions. Following a biomimetic approach, we have engineered fully synthetic nanoparticles that are able to self-organize their surface into controlled domains. We focused on Polymeric vesicles or "polymersomes"; enclosed membranes formed via self-assembly,of amphiphilic block copolymers In water. Exploiting the Intrinsic thermodynamic tendency of dissimilar polymers to undergo phase separation, we mixed different vesicle forming block copolymers In various proportions In order to obtain a wide range of polymersomes with differing surface domains. Using a combination of confocal laser. scanning microscopy studies of micrometer-sized polymersomes, and electron microscopy, atomic force microscopy, and fluorescence spectroscopy on nanometer-sized polymersomes, we find that the domains exhibit similar shapes on both the micro- and nanolength scales, with dimensions that are linearly proportional to the vesicle diameter. Finally, we demonstrate that such control over the surface "patchiness" of these polymersomes determines their cell internalization kinetics for live cells.