Effects of Porphyromonas gingivalis infection on human gingival epithelial barrier function in vitro
Effects of Porphyromonas gingivalis infection on human gingival epithelial barrier function in vitro
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DOI：
10.1111/j.1600-0722.2010.00782.x
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发表时间：
2010-12-01
影响因子：
1.9
通讯作者：
Meyle, Joerg
中科院分区：
文献类型：
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作者：
Groeger, Sabine;Doman, Eugen;Meyle, Joerg
牙龈上皮在保护口腔组织免受微生物攻击方面起着重要作用。口腔角质形成细胞形成各种细胞接触，包括紧密连接，因此能够形成一种上皮屏障。体外屏障功能的一个可测量的指标是跨上皮电阻(TER)。牙龈卟啉单胞菌是目前公认的牙周病的主要病原菌，具有多种致病因子。本研究的目的是在体外研究牙龈假单胞菌感染对由原代和永生化的人角质形成细胞组成的牙龈屏障的影响。原代和永生化的人牙龈角质形成细胞感染不同菌株的牙龈假单胞菌。通过测定TER来分析细菌挑战对屏障的影响。牙龈痛的破坏性作用可被特定的酶抑制剂阻断。在感染井中最初增加约20%-30%后，TER降至零。姜黄素抑制剂使屏障的破坏延迟了12+/-4h。在所有病例中，如果系统从基底外侧感染，TER的丢失都会加速。牙龈假单胞菌对三维培养上皮细胞模型的上皮屏障功能有明显的影响，这部分可以归因于牙龈痛的活性。
The gingival epithelium plays an important role in the protection of oral tissues from microbial challenge. Oral keratinocytes form various cellular contacts, including tight junctions, and thus are able to create an epithelial barrier. A measurable indicator of barrier function in vitro is the transepithelial electrical resistance (TER). Porphyromonas gingivalis is recognized as a major aetiologic agent of periodontal disease and exhibits a variety of virulence factors. The aim of the study was to investigate the effect, in vitro, of infection with P. gingivalis on gingival barriers composed of primary and immortalized human keratinocytes. Primary and immortalized human gingival keratinocytes were infected with different strains of P. gingivalis. The impact of the bacterial challenge on the barrier was analysed by measuring the TER. The destructive effects of gingipains were blocked by specific enzyme inhibitors. After an initial increase of about 20-30% in infected wells, the TER decreased to zero. Gingipain inhibitors delayed the destruction of the barrier by 12 +/- 4 h. In all cases, the loss of TER was accelerated if the system was infected from the basolateral side. A distinct effect of P. gingivalis on the epithelial barrier function of three-dimensional cultured epithelial cell models was demonstrated, which can partly be attributed to the activity of gingipains.