Bacterial diversity in saliva and oral health-related conditions: the Hisayama Study.
Bacterial diversity in saliva and oral health-related conditions: the Hisayama Study.
复制标题
DOI：
10.1038/srep22164
复制
发表时间：
2016-02-24
影响因子：
4.6
通讯作者：
Yamashita Y
中科院分区：
文献类型：
--
作者：
Takeshita T;Kageyama S;Furuta M;Tsuboi H;Takeuchi K;Shibata Y;Shimazaki Y;Akifusa S;Ninomiya T;Kiyohara Y;Yamashita Y
这项基于人群的研究使用16S rRNA基因下一代高通量测序确定了日本Hisayama镇2，343名成年居民的唾液微生物群组成。在550个已确定的物种水平操作分类单位（OTU）中，有72个是常见的，占所有个体的≥75%，以及在系统发育多样性（PD）最低的五分位数中的≥75%。这些“核心”OTU构成每个微生物组的90.9 ± 6.1%。对平均相对丰度≥1%的22个核心OTU的相对丰度剖面进行了划分，将其划分为群落类型I和群落类型II。多元回归分析显示，较低的PD与更好的口腔健康状况相关，包括较低的菌斑指数，没有蛀牙，牙龈出血较少，牙周袋较浅，不吸烟，也与牙齿脱落有关。相比之下，多重泊松回归分析表明，社区类型II，其特征在于较高的比例的9个占主导地位的核心OTU，包括黄色奈瑟氏菌，涉及年轻，较低的身体质量指数，较少的牙齿与龋齿的经验，不吸烟。我们的大规模数据分析揭示了日本成年人唾液微生物组的变化以及与唾液微生物组相关的口腔健康相关疾病。
This population-based study determined the salivary microbiota composition of 2,343 adult residents of Hisayama town, Japan, using 16S rRNA gene next-generation high-throughput sequencing. Of 550 identified species-level operational taxonomic units (OTUs), 72 were common, in ≥75% of all individuals, as well as in ≥75% of the individuals in the lowest quintile of phylogenetic diversity (PD). These “core” OTUs constituted 90.9 ± 6.1% of each microbiome. The relative abundance profiles of 22 of the core OTUs with mean relative abundances ≥1% were stratified into community type I and community type II by partitioning around medoids clustering. Multiple regression analysis revealed that a lower PD was associated with better conditions for oral health, including a lower plaque index, absence of decayed teeth, less gingival bleeding, shallower periodontal pockets and not smoking, and was also associated with tooth loss. By contrast, multiple Poisson regression analysis demonstrated that community type II, as characterized by a higher ratio of the nine dominant core OTUs, including Neisseria flavescens, was implicated in younger age, lower body mass index, fewer teeth with caries experience, and not smoking. Our large-scale data analyses reveal variation in the salivary microbiome among Japanese adults and oral health-related conditions associated with the salivary microbiome.