Spectral fluorescence lifetime detection and selective melanin imaging by multiphoton laser tomography for melanoma diagnosis
Spectral fluorescence lifetime detection and selective melanin imaging by multiphoton laser tomography for melanoma diagnosis
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DOI：
10.1111/j.1600-0625.2008.00815.x
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发表时间：
2009-06-01
影响因子：
3.6
通讯作者：
Kaatz, Martin
中科院分区：
文献类型：
--
作者：
Dimitrow, Enrico;Riemann, Iris;Kaatz, Martin
多光子激发的组织荧光概括了所有天然存在的内源性荧光生物分子的发射，其荧光光谱通常重叠。普通的荧光强度测量不能用于区分不同的荧光团或代谢状态。为了克服这一局限性，我们研究了选择性黑色素成像和光谱荧光寿命成像结合高分辨率多光子激光断层扫描的新程序。总共分析了46个人皮肤的黑素细胞病变。我们认为，以这种方式检测到的荧光可能会为黑色素瘤诊断提供额外的信息。观察到角质形成细胞与黑素细胞在寿命行为上的显着差异。荧光寿命分布与细胞内黑色素含量相关。黑色素瘤的光谱分析揭示了在470 nm附近的主要荧光峰与遍及所有表皮层的接近550 nm的附加峰的组合。在800 nm处的激发显示了含有黑色素的细胞的选择性可观察的荧光，并提供了细胞分类的可能性。选择性成像以及光谱荧光寿命成像的程序，通过多光子激光断层扫描支持诊断决策，并可能改善黑色素瘤的非侵入性早期检测的过程。
Multiphoton excited tissue fluorescence summarises the emission of all naturally occurring endogenous fluorescent bio-molecules with their often overlapping fluorescence spectra. Common fluorescence intensity measurements could not be utilised to distinguish between different fluorophores or metabolic states. To overcome this limitation, we investigated new procedures of selective melanin imaging and spectral fluorescence lifetime imaging in combination with high resolution multiphoton laser tomography. Overall 46 melanocytic lesions of human skin were analysed. We suggested that fluorescence light, detected in such a way, may yield additional information for melanoma diagnostics. Remarkable differences in lifetime behaviour of keratinocytes in contrast to melanocytes were observed. Fluorescence lifetime distribution was found in correlation with the intracellular amount of melanin. Spectral analysis of melanoma revealed a main fluorescence peak around 470 nm in combination with an additional peak close to 550 nm throughout all epidermal layers. Excitation at 800 nm shows a selectively observable fluorescence of melanin containing cells and offers the possibility of cell classification. Procedures of selective imaging as well as spectral fluorescence lifetime imaging by means of multiphoton laser tomography support diagnostic decisions and may improve the process of non-invasive early detection of melanoma.