Enhancement of wound healing by hyperbaric oxygen and transforming growth factor β3 in a new chronic wound model in aged rabbits
Enhancement of wound healing by hyperbaric oxygen and transforming growth factor β3 in a new chronic wound model in aged rabbits
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DOI：
10.1001/archsurg.135.10.1148
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发表时间：
2000-10-01
影响因子：
--
通讯作者：
Mustoe, TA
中科院分区：
文献类型：
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作者：
Bonomo, SR;Davidson, JD;Mustoe, TA
假设：虽然高压氧(HBO)在临床上已经使用了30年，但很少有对照临床试验。动物模型还不足以测试高压氧单独或与生长因子联合治疗慢性伤口的疗效。我们假设在一种新的缺血性慢性创伤动物模型中，高压氧与原型生长因子一样有效地促进创面愈合。设计：25只老年兔和3只年轻兔的耳朵呈现慢性缺血性，并造成软骨水平的溃疡。这些溃疡的治疗方法有三种：HBO治疗，每天90分钟，从周一到周五，持续3周；转化生长因子β(3)1微克/厘米(2)；或两者结合使用。对照组用车内空气或高压室空气，或两者兼用。结果：该模型造成的老年/缺血创面在伤后数天内不能自动愈合(与老年/非缺血对照相比减少88%)。单独使用高压氧和单独使用转化生长因子β(3)均可提高愈合率(与老年/非缺血对照组相比，愈合率仅降低38%)。结论：在老年动物中，高压氧和转化生长因子β(3)在促进创面愈合方面同样有效，我们的数据表明，高压氧单独用于慢性创面可能比在急性创面更有效。在幼兔身上，在相同的伤口模型中，综合治疗方法没有额外的益处。
Hypothesis: Although hyperbaric oxygen (HBO) has been used clinically for 3 decades, there have been few controlled clinical trials. Animal models have not been adequate to test the efficacy of HBO in the treatment of chronic wounds, either by itself or in combination with growth factors. We hypothesize that HBO is as efficacious as a prototype growth factor in improving wound healing in a new animal model of ischemic chronic wounds.Design: Twenty-five aged rabbits and 3 young rabbits had their ears rendered chronically ischemic and ulcers were created down to the level of cartilage. These ulcers were treated in 1 of 3 ways: with HBO, 90 minutes per day, Monday through Friday, for 3 weeks; with transforming growth factor beta(3) at 1 mu g/cm(2); or with both modalities combined. Controls were treated with vehicle or hyperbaric room air or both.Results: This model created an aged/ischemic wound that failed to heal spontaneously up to LO; days after wounding (88% reduction compared with aged/nonischemic controls). Hyperbaric oxygen alone and transforming growth factor beta(3) alone both improved healing rate (only 38% reduction in healing compared with aged/nonischemic controls). Combined therapy produced no additional improvement over either modality by itself.Conclusions: In aged animals, HBO and transforming growth factor beta(3) were equally effective in improving wound healing, Our data suggest that HBO alone may be more effective in the chronic wound than in the acute wound. There was no additive benefit to combining modalities as has been reported in the same wound model in young rabbits.