Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor
Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor
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DOI：
10.1016/j.jfca.2012.02.004
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发表时间：
2012-05-01
影响因子：
4.3
通讯作者：
Van Griensven, Leo J. L. D.
中科院分区：
文献类型：
--
作者：
Kozarski, Maja;Klaus, Anita;Van Griensven, Leo J. L. D.
本文研究了四种最广为人知的蘑菇的多糖提取物的抗氧化活性，这些蘑菇通常用于药用、茶和食品中，即灵芝、灵芝、香菇和色曲菌。白莲子和白莲子提取物对DPPH的清除作用最强，EC50 < 0.1 mg/mL。扁木提取物对亚铁离子的螯合能力最强（EC50 = 3.58 mg/mL），对脂质过氧化的抑制作用最强（EC50 = 2.07 mg/mL），还原能力最强（EC50 = 0.18）。四种多糖提取物的还原力与总酚和α -葡聚糖含量有较强的相关性，而与总多糖和总蛋白质含量无明显相关性。提取物含有多糖、蛋白质和多酚的混合物/复合物，经热水处理、乙醇沉淀和透析后仍存在。葡萄糖是所有多糖中普遍存在的单糖，样品中含有α -葡聚糖和β -葡聚糖。总葡聚糖含量变化很大，从扁桃的35%到灵芝的83%。在板栗多糖中发现了糖醛酸。傅里叶变换红外（FT-IR）分析显示，在不同糖和多糖信号旁有较强的芳香和酯峰。(C) 2012爱思唯尔公司版权所有。
Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts were the strongest DPPH scavengers, i.e. EC50 < 0.1 mg/mL. G. applanatum extract had the highest chelating ability of ferrous ion (EC50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC50 = 2.07 mg/mL) and had the highest reducing power, EC50 = 0.18. All four polysaccharide extracts showed a strong correlation between the reducing power and the total amount of phenols and alpha-glucans: no such correlation was found between the reducing power and the amount of total polysaccharides and proteins. The extracts contained a mixture/complex of polysaccharides, proteins and polyphenols, still present after hot water treatment, ethanol precipitation and dialysis. Glucose was the prevalent monosaccharide of all polysaccharides and samples contained both alpha- and beta-glucans. Total glucan contents varied considerably, i.e. from 35% for G. applanatum to 83% for G. lucidum. Uronic acid was found in the G. applanatum polysaccharides. Fourier transform infrared (FT-IR) analysis showed strong aromatic and ester peaks next to the different sugar and polysaccharide signals. (C) 2012 Elsevier Inc. All rights reserved.