Comprehensive gene expression analysis of rice aleurone cells: probing the existence of an alternative gibberellin receptor
Comprehensive gene expression analysis of rice aleurone cells: probing the existence of an alternative gibberellin receptor
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水稻糊粉细胞的综合基因表达分析：探讨替代赤霉素受体的存在
DOI：
10.1104/pp.114.247940
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发表时间：
2015
期刊：
影响因子：
7.4
通讯作者：
Makoto Matsuoka
中科院分区：
文献类型：
--
作者：
Kenji Yano;Koichiro Aya;Ko Hirano;Reynante Laesamana Ordonio;Miyako Ueguchi- Tanaka;Makoto Matsuoka
赤霉素（GA）的研究表明，GA必须在植物细胞核中被其同源受体GIBBERELLIN INSENSITIVE DWARF 1（GID 1）所感知。通过GID 1识别GA可以解除DELLA蛋白介导的抑制，这是一种称为GID 1-DELLA GA感知系统的模型。已经有报道在质膜中存在潜在的GA结合蛋白，其感知GA并诱导谷物糊粉细胞中的α-淀粉酶表达，其机制不同于GID 1-DELLA系统。因此，我们检测了GA受体（gid 1）和DELLA阻遏物（slr 1）受损的水稻突变体中水稻（Oryza sativa）α-淀粉酶基因的表达，并证实了它们对GA ingid 1突变体缺乏应答，而在slr 1突变体中组成型表达。我们还研究了GA调节基因的表达，通过全基因组微阵列和定量逆转录聚合酶链反应分析，并证实所有GA调节基因的调控由GID 1-DELLA系统。此外，我们还利用一组GA感知和基因表达相关基因缺陷的突变体，即lygid 1、slr 1、gid 2（GA相关的F-box蛋白突变体）和gamyb（GA相关的反式作用因子突变体），研究了GA信号转导的调控网络。几乎所有的GA上调基因都受到这四种GA信号组分的调控。另一方面，GA下调的基因相对于GID 2和GAMYB显示出不同的表达模式（例如，相当多的基因不受GAMYB或GID 2和GAMYB控制）。基于这些观察结果，我们提出了一个全面的讨论，在水稻糊粉细胞中的GA调控基因的复杂网络。
Current gibberellin (GA) research indicates that GA must be perceived in plant nuclei by its cognate receptor, GIBBERELLIN INSENSITIVE DWARF1 (GID1). Recognition of GA by GID1 relieves the repression mediated by the DELLA protein, a model known as the GID1-DELLA GA perception system. There have been reports of potential GA-binding proteins in the plasma membrane that perceive GA and induce α-amylase expression in cereal aleurone cells, which is mechanistically different from the GID1-DELLA system. Therefore, we examined the expression of the rice (Oryza sativa) α-amylase genes in rice mutants impaired in the GA receptor (gid1) and the DELLA repressor (slender rice1; slr1) and confirmed their lack of response to GA ingid1mutants and constitutive expression inslr1mutants. We also examined the expression of GA-regulated genes by genome-wide microarray and quantitative reverse transcription-polymerase chain reaction analyses and confirmed that all GA-regulated genes are modulated by the GID1-DELLA system. Furthermore, we studied the regulatory network involved in GA signaling by using a set of mutants defective in genes involved in GA perception and gene expression, namelygid1,slr1,gid2(a GA-related F-box protein mutant), andgamyb(a GA-related trans-acting factor mutant). Almost all GA up-regulated genes were regulated by the four named GA-signaling components. On the other hand, GA down-regulated genes showed different expression patterns with respect to GID2 and GAMYB (e.g. a considerable number of genes are not controlled by GAMYB or GID2 and GAMYB). Based on these observations, we present a comprehensive discussion of the intricate network of GA-regulated genes in rice aleurone cells.