Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter
Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter
复制标题
野生北海道梅花鹿瘤胃细菌菌群的季节差异以及可能参与冬季纤维消化的未知细菌群的部分特征
DOI：
10.1111/asj.13203
复制
发表时间：
2019
影响因子：
2
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Yamano;H.;Ichimura;Y.;Sawabe;Y.;Koike;S.;Suzuk;Y.;Kobayashi;Y.
对野生北海道梅花鹿进行瘤胃消化，比较夏季和冬季的细菌区系。细菌区系特征采用分子生物学方法和增菌培养方法，类杆菌为主要类群，其次是类杆菌，在两个季节的比例相似。然而，在每个季节都发现了两个独特的类杆菌类群：冬季的未知类群A和夏季的未知类群B。未知A组冬季瘤胃丰度最高，其次为黄色瘤胃球菌。此外，两者的丰度在冬季高于夏季。相比之下，未知B类群夏季的丰度高于冬季。此外，在量化的细菌中，这一组在夏季表现出最高的丰度。用橡树皮和无菌瘤胃液，特别是鹿的瘤胃液培养，成功地丰富了未知的A组。该组细菌主要分布在固体而不是液体瘤胃中，并以小球菌的形式存在。因此，假设未知基团A参与纤维材料的降解。这些结果表明，野生北海道梅花鹿会对饮食条件的变化产生瘤胃细菌菌群。
Rumen digesta was obtained from wild Hokkaido sika deer to compare bacterial flora between summer and winter. Bacterial flora was characterized with molecular‐based approaches and enrichment cultivation.Bacteroideteswas shown as a major phylum followed byFirmicutes, with similar proportions in both seasons. However, two phylogenetically unique groups inBacteroideteswere found in each season: unknown group A in winter and unknown group B in summer. The ruminal abundance of unknown group A was the highest followed byRuminococcus flavefaciensin winter. Moreover, the abundance of these two was higher in winter than in summer. In contrast, the abundance of unknown group B was higher in summer than in winter. In addition, this group showed the highest abundance in summer among the bacteria quantified. Unknown group A was successfully enriched by cultivating with oak bark and sterilized rumen fluid, particularly that from deer. Bacteria of this group were distributed in association with the solid rather than the liquid rumen fraction, and were detected as small cocci. Accordingly, unknown group A is assumed to be involved in degradation of fibrous materials. These results suggest that wild Hokkaido sika deer develop a rumen bacterial flora in response to changes in dietary conditions.