The sensitivity and heterogeneity of histochemical markers for altered foci involved in liver carcinogenesis.
The sensitivity and heterogeneity of histochemical markers for altered foci involved in liver carcinogenesis.
复制标题
肝癌发生过程中改变病灶的组织化学标记物的敏感性和异质性。
DOI：
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发表时间：
1979
影响因子：
6
通讯作者：
G. Williams
中科院分区：
文献类型：
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作者：
N. Hirota;G. Williams
皮下注射右旋糖酐铁导致大鼠在2周内出现肝铁质沉着症，与先前对小鼠的报道相同。大鼠的肝癌以及肿瘤结节完全或主要不含铁，因此，可以很容易地在组织学上识别。此外，早期致癌物诱导的改变灶对铁积累有抵抗力。在喂食0.02%N-2-芴基乙酰胺（FAA）13周的大鼠中，铁注射后确定的铁耐药灶的数量与膳食铁过载时观察到的数量相同。已被用来确定病灶的酶标记物的组织化学研究表明，病灶的特点是酶反应阳性γ-谷氨酰转肽酶和减少腺苷三磷酸酶和葡萄糖-6-磷酸酶对应的特点是耐铁积累。然而，在定量分析的早期致癌物诱导的病灶在大鼠给予铁右旋糖酐后的饮食含有0.02%2-FAA的13周，更多的病变检测到铁积累的阻力比任何这些其他属性。有相当大的表型异质性焦点之间的酶标记物。它的结论是，抵抗铁积累是一个更敏感和可靠的早期致癌物引起的肝细胞病灶改变的标志物比任何其他组织化学性质。
Subcutaneous injection of iron dextran resulted in a hepatic siderosis within 2 weeks in rats, as previously reported for mice. Hepatic carcinomas as well as neoplastic nodules in rats were entirely or mainly free of stainable iron and, thus, could be readily identified histologically. In addition, early carcinogen-induced altered foci were resistant to iron accumulation. In rats fed 0.02% N-2-fluorenylacetamide (FAA) for 13 weeks, the number of iron-resistant foci identified following iron injection was the same as that observed with dietary iron overload. Histochemical investigation of enzymatic markers that have been used to identify foci in rats revealed that foci characterized by enzymatic reactions of positive gamma-glutamyl transpeptidase and decreased adenosine triphosphatase and glucose-6-phosphatase corresponded to those characterized by resistance to iron accumulation. However, in quantitative analysis of the early carcinogen-induced foci in rats given iron dextran following a diet containing 0.02% 2-FAA for 13 weeks, more lesions were detected by resistance to iron accumulation than by any of these other properties. There was considerable phenotypic heterogeneity among foci for the enzyme markers. It is concluded that resistance to iron accumulation is a more sensitive and reliable marker for early carcinogen-induced altered hepatocellular foci than is any other histochemical property.