Tracing Changes in the Microbial Community of a Hydrocarbon-Polluted Soil by Culture-Dependent Proteomics
Tracing Changes in the Microbial Community of a Hydrocarbon-Polluted Soil by Culture-Dependent Proteomics
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DOI：
10.1016/s1002-0160(10)60037-9
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发表时间：
2010-08-01
期刊：
影响因子：
5.7
通讯作者：
Garcia, C.
中科院分区：
文献类型：
--
作者：
Bastida, F.;Nicolas, C.;Garcia, C.
碳氢化合物污染可能从多样性和功能两方面影响土壤微生物群落。建立了半干旱对照土壤和人工柴油污染土壤的实验室实验，通过蛋白质组学方法跟踪碳氢化合物污染土壤中微生物群落优势种的变化。从Luria-Bertani(Luria-Bertani，Luria-Bertani大多数蛋白质与糖酵解途径、结构或蛋白质合成有关。结果表明，土壤微生物群落的复杂性随着碳氢化合物污染的增加而相对增加，尤其是在培养15天后。青枯拉尔氏菌、长聚球藻和不同的梭状芽孢杆菌等。在对照土壤中没有出现，但芽孢杆菌属(Bacillussp.)在任何一种处理中，都主导了兰州的生长。我们认为，用依赖于培养的蛋白质组学方法鉴定土壤提取物中的微生物种类可以部分解释碳氢化合物污染的半干旱土壤微生物群落多样性的变化，但这一信息比分子方法提供的信息要有限得多。
Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani (LB) media showed a change in the microbial community. The majority of the proteins were related to glycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.