A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology
A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology
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DOI：
10.1016/s0264-410x(98)80123-0
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发表时间：
1998-07-01
期刊：
影响因子：
5.5
通讯作者：
Singer, RS
中科院分区：
文献类型：
--
作者：
Gershwin, LJ;Schelegle, ES;Singer, RS
一个关键问题是观察到，儿童接种福尔马林灭活呼吸道合胞病毒(RSV)疫苗与疾病增加有关。我们利用牛RSV和我们在小牛身上对这种疾病的经验开发了一个类似于人类疾病的自然模型，使用福尔马林灭活的牛RSV疫苗，小牛要么假接种/感染，要么接种/感染，要么。接种疫苗/假感染，并对他们的临床体征、肺功能和肺组织学损害进行量化评分。有趣的是，接种/感染的小牛的疾病显著增加，小牛的组织学损害与感染儿童相似。最后，我们注意到，接种疫苗没有诱导中和抗体，但通过ELISA检测到了抗体。我们的RSV增强型疾病模型是重要的，因为它提供了疾病严重程度的量化证据，可用于评估免疫病理机制和候选RSV疫苗的安全性。(C)1998爱思唯尔科学有限公司，保留所有权利。
A critical issue has been the observation that vaccination of children with a formalin-inactivated respiratory syncytial vines (RSV) vaccine is associated with disease enhancement. We have taken advantage of bovine RSV and our experience with this disease in calves to develop a natural model that parallels human disease, Using formalin-inactivated bovine RSV vaccine calves were either sham-vaccinated/infected, vaccinated/infected, or. vaccinated/sham-infected and their clinical signs, pulmonary function, and histological lung lesions quantitatively scored. interestingly there was significantly greater disease in vaccinated/infected calves and histological lesions in calves were similar to those of affected children, Finally, we note that vaccination did not induce neutralizing antibodies, bur Ige antibodies were detected by ELISA, Our model of RSV enhanced disease is important because it provides quantifiable evidence of disease severity that can be applied to evaluate the mechanisms of immunopathology and the safety of candidate RSV vaccines. (C) 1998 Elsevier Science Ltd, All rights reserved.