A Simple low-cost device enables four epi-illumination techniques on standard light microscopes.
A Simple low-cost device enables four epi-illumination techniques on standard light microscopes.
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一种简单的低成本设备可在标准光显微镜上采用四种表弹技术。
DOI：
10.1038/srep20729
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发表时间：
2016-02-08
影响因子：
4.6
通讯作者：
Berry RM
中科院分区：
文献类型：
--
作者：
Ishmukhametov RR;Russell AN;Wheeler RJ;Nord AL;Berry RM
后向散射暗场（BSDF）、落射荧光（EF）、干涉反射对比（IRC）和暗场表面反射（DFSR）是先进但昂贵的光学显微镜技术，其可用性有限。在这里，我们展示了一个简单的光学设计，结合了这四种技术在一个简单的低成本的微型落射照明器，插入到差分干涉对比度（DIC）滑块湾的商业显微镜，而无需进一步添加。我们使用该设备证明：1）基于BSDF的未染色人红细胞内疟疾寄生虫的检测; 2）具有和不具有二色性组分的EF成像，包括在不使用激发或发射滤光片的情况下检测DAPI染色的利什曼原虫寄生虫; 3）黑色脂质膜和其他薄膜的RIC，以及4）图案化不透明和透明表面的DFSR。我们相信，我们的设计可以扩展商业明场显微镜的功能，提供简单的寄生虫现场检测和感兴趣的许多用户的光学显微镜。
Back-scattering darkfield (BSDF), epi-fluorescence (EF), interference reflection contrast (IRC), and darkfield surface reflection (DFSR) are advanced but expensive light microscopy techniques with limited availability. Here we show a simple optical design that combines these four techniques in a simple low-cost miniature epi-illuminator, which inserts into the differential interference-contrast (DIC) slider bay of a commercial microscope, without further additions required. We demonstrate with this device: 1) BSDF-based detection of Malarial parasites inside unstained human erythrocytes; 2) EF imaging with and without dichroic components, including detection of DAPI-stained Leishmania parasite without using excitation or emission filters; 3) RIC of black lipid membranes and other thin films, and 4) DFSR of patterned opaque and transparent surfaces. We believe that our design can expand the functionality of commercial bright field microscopes, provide easy field detection of parasites and be of interest to many users of light microscopy.