Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties.
Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties.
复制标题
细胞渗透性肽核酸的合成及其杂交和摄取特性的表征。
DOI：
10.1016/j.bmcl.2006.06.052
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Ly,DanithH
中科院分区：
文献类型：
--
作者：
Zhou,Peng;Dragulescu-Andrasi,Anca;Bhattacharya,Birendra;O'Keefe,Heather;Vatta,Paolo;Hyldig-Nielsen,JensJ;Ly,DanithH
合成了含有四种天然碱基(腺嘌呤(A)、胞嘧啶(C)、鸟嘌呤(G)和胸腺嘧啶(T))和两种非天然碱基(2-硫尿嘧啶(SU)和2，6-二氨基嘌呤(D))的胍基肽核酸(GPNA)单体和低聚物。热变性研究表明，含有交替D-骨架构型的GPNA寡聚体与DNA特异性结合，当与哺乳动物细胞孵育时，特异性定位于内质网(ER)。
Guanidine-based peptide nucleic acid (GPNA) monomers and oligomers containing all four natural (adenine (A), cytosine (C), guanine (G), and thymine (T)) and two unnatural (2-thiouracil (sU) and 2,6-diaminopurine (D)) nucleobases have been synthesized. Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when incubated with mammalian cells, localized specifically to the endoplasmic reticulum (ER).