The rate and character of spontaneous mutation in an RNA virus.
The rate and character of spontaneous mutation in an RNA virus.
复制标题
RNA 病毒自发突变的速率和特征。
DOI：
10.1093/genetics/162.4.1505
复制
发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Garcia-Arenal,Fernando
中科院分区：
文献类型：
--
作者：
Malpica,JoseM;Fraile,Aurora;Moreno,Ignacio;Obies,ClaraI;Drake,JohnW;Garcia-Arenal,Fernando
对RNA病毒自发突变率的估计很少且不确定，最显著的原因是它们依赖于可能不能很好地代表整个基因组的微小突变报告序列。我们在这里报告的估计自发突变率的烟草花叶病毒使用804碱基同源突变的目标，viralMP基因编码的运动蛋白（MP）。通过提供来自转基因的MP功能来对抗针对新出现的突变体的选择。估计的基因组突变率在先前估计的裂解性动物核糖病毒范围的较低侧。我们还提出了第一个公正的核糖病毒突变谱。碱基替换的比例与逆转录病毒相同，但低于大多数基于DNA的生物体。虽然MP突变频率为0.02-0.05，但35%的测序突变体含有两个或两个以上的突变。因此，TMV和核糖病毒群体中的突变过程通常与基于DNA的微生物群体中的突变过程有很大不同，并且可能受到增变聚合酶亚群的强烈影响。
Estimates of spontaneous mutation rates for RNA viruses are few and uncertain, most notably due to their dependence on tiny mutation reporter sequences that may not well represent the whole genome. We report here an estimate of the spontaneous mutation rate of tobacco mosaic virus using an 804-base cognate mutational target, the viralMPgene that encodes the movement protein (MP). Selection against newly arising mutants was countered by providing MP function from a transgene. The estimated genomic mutation rate was on the lower side of the range previously estimated for lytic animal riboviruses. We also present the first unbiased riboviral mutational spectrum. The proportion of base substitutions is the same as that in a retrovirus but is lower than that in most DNA-based organisms. Although theMPmutant frequency was 0.02-0.05, 35% of the sequenced mutants contained two or more mutations. Therefore, the mutation process in populations of TMV and perhaps of riboviruses generally differs profoundly from that in populations of DNA-based microbes and may be strongly influenced by a subpopulation of mutator polymerases.