Differential Impact of Ad Libitum or Intermittent High-Fat Diets on Bingeing Ethanol-Mediated Behaviors
Differential Impact of Ad Libitum or Intermittent High-Fat Diets on Bingeing Ethanol-Mediated Behaviors
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DOI：
10.3390/nu11092253
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发表时间：
2019-09-01
期刊：
影响因子：
5.9
通讯作者：
Rodriguez-Arias, Marta
中科院分区：
文献类型：
--
作者：
Del Olmo, Nuria;Carmen Blanco-Gandia, M.;Rodriguez-Arias, Marta
背景：饮食因素对大脑、调节情绪、焦虑、动机和认知有显著影响。到目前为止，乙醇(Etoh)和高脂饮食(HFD)的组合对青春期学习和情绪障碍的影响还没有引起注意。本工作的目的是评估酗酒和高脂饮食对青春期小鼠的生化和行为后果。方法：动物接受标准饮食或HFD(随意饮酒与酗酒模式)，并对焦虑和记忆进行评估。并对瘦素受体和时钟基因的代谢谱和基因表达进行了研究。结果：自由活动的HFD+ETOH小鼠主要表现为白色脂肪组织增多，血浆胰岛素和瘦素水平升高。临时性HFD组大鼠前额叶皮质和海马区Lepr基因表达上调。在暴露于HFD的小鼠中，乙醇诱导的空间记忆提取障碍是不存在的，尽管厌恶的记忆缺陷仍然存在。暴饮暴食HFD的小鼠只表现出一种缓解焦虑的特征，没有其他变化。我们还观察到，在随意的HFD动物中，CLOCK和BMal1的表达存在不匹配，这基本上与Etoh结合无关。结论：我们的结果证实了高脂饮食的成分和摄入模式与青春期酒精摄入量之间的双向影响，即使这种相互作用的代谢、行为和时间生物学影响是分离的。
Background: Dietary factors have significant effects on the brain, modulating mood, anxiety, motivation and cognition. To date, no attention has been paid to the consequences that the combination of ethanol (EtOH) and a high-fat diet (HFD) have on learning and mood disorders during adolescence. The aim of the present work was to evaluate the biochemical and behavioral consequences of ethanol binge drinking and an HFD consumption in adolescent mice. Methods: Animals received either a standard diet or an HFD (ad libitum vs. binge pattern) in combination with ethanol binge drinking and were evaluated in anxiety and memory. The metabolic profile and gene expression of leptin receptors and clock genes were also evaluated. Results: Excessive white adipose tissue and an increase in plasma insulin and leptin levels were mainly observed in ad libitum HFD + EtOH mice. An upregulation of the Lepr gene expression in the prefrontal cortex and the hippocampus was also observed in ad libitum HFD groups. EtOH-induced impairment on spatial memory retrieval was absent in mice exposed to an HFD, although the aversive memory deficits persisted. Mice bingeing on an HFD only showed an anxiolytic profile, without other alterations. We also observed a mismatch between Clock and Bmal1 expression in ad libitum HFD animals, which were mostly independent of EtOH bingeing. Conclusions: Our results confirm the bidirectional influence that occurs between the composition and intake pattern of a HFD and ethanol consumption during adolescence, even when the metabolic, behavioral and chronobiological effects of this interaction are dissociated.