THE HUMAN BRADYKININ B-2 RECEPTOR GENE - FULL-LENGTH CDNA, GENOMIC ORGANIZATION AND IDENTIFICATION OF THE REGULATORY REGION
THE HUMAN BRADYKININ B-2 RECEPTOR GENE - FULL-LENGTH CDNA, GENOMIC ORGANIZATION AND IDENTIFICATION OF THE REGULATORY REGION
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DOI：
10.1006/bbrc.1995.1800
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发表时间：
1995-06-06
影响因子：
3.1
通讯作者：
ROSCHER, AA
中科院分区：
文献类型：
--
作者：
KAMMERER, S;BRAUN, A;ROSCHER, AA
北方印迹与相应于缓激肽B-2受体编码区的探针杂交，显示约4.0 kb的单个转录物。使用相同的探针，我们检测到一个2.6 kb的人cDNA克隆，该克隆与已知编码区的3'端重叠103个碱基对，并含有一个经典的多聚腺苷酸化位点和一个polyA尾。人B-2受体的基因分离自人胎盘基因组文库。对几个基因组λ克隆的分析表明，B-2受体基因由三个外显子组成。基因组Southern分析显示B-2受体由单拷贝基因编码。原位杂交结果表明该基因位于染色体14 q32。通过在荧光素酶报告基因分析中测试不同的推定调控片段，证实了外显子1的5'上游区的基因激活功能。(C)出版社：Academic Press
Hybridization of Northern blots with a probe corresponding to the coding region of the bradykinin B-2 receptor revealed a single transcript of approximately 4.0 kb. Using the same probe, we detected a 2.6 kb human cDNA clone that overlapped 103 bp with the 3' end of the known coding region and contained a classical polyadenylation site and a polyA tail. The gene for the human B-2 receptor was isolated from a human placenta genomic library. Analysis of several genomic lambda clones indicated that the B-2 receptor gene is organized in three exons. Genomic Southern analysis revealed the B-2 receptor to be encoded by a single copy gene. In situ hybridization showed that the gene is located on chromosome 14q32. By testing different putative regulatory fragments in a luciferase reporter assay, a gene activating function of the 5' upstream region of exon 1 was demonstrated. (C) 1995 Academic Press, Inc.