The postsynaptic density proteins Homer and Shank form a polymeric network structure.
The postsynaptic density proteins Homer and Shank form a polymeric network structure.
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DOI：
10.1016/j.cell.2009.01.050
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发表时间：
2009-04-03
期刊：
影响因子：
64.5
通讯作者：
Hayashi Y
中科院分区：
文献类型：
--
作者：
Hayashi MK;Tang C;Verpelli C;Narayanan R;Stearns MH;Xu RM;Li H;Sala C;Hayashi Y
突触后密度（PSD）是突触功能的关键，但保持其结构和成分的分子结构仍然是难以捉摸的。Homer和Shank是PSD中最丰富的支架蛋白，协同作用使树突棘成熟。在这里，我们证明，荷马和柄，一起，形成一个网状矩阵结构。该区域的晶体学分析揭示了一对平行的二聚体卷曲螺旋以尾对尾的方式插入以形成四聚体，从而在卷曲螺旋的每个末端产生一对氨基末端EVH 1结构域的独特构型。在神经元中，树突棘的结构完整性和蛋白质向突触的募集需要四聚体。我们建议，Homer-Shank复合物作为一个结构框架，并作为其他PSD蛋白的组装平台。
The postsynaptic density (PSD) is crucial for synaptic functions, but the molecular architecture retaining its structure and components remains elusive. Homer and Shank are among the most abundant scaffolding proteins in the PSD, working synergistically for maturation of dendritic spines. Here, we demonstrate that Homer and Shank, together, form a mesh-like matrix structure. Crystallographic analysis of this region revealed a pair of parallel dimeric coiled-coils intercalated in a tail-to-tail fashion to form a tetramer, giving rise to the unique configuration of a pair of amino-terminal EVH1 domains at each end of the coiled-coil. In neurons, the tetramerization is required for structural integrity of the dendritic spines and recruitment of proteins to synapses. We propose that the Homer-Shank complex serves as a structural framework and as an assembly platform for other PSD proteins.
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