Transcriptional activation of HIV by Mycobacterium tuberculosis in human monocytes.
Transcriptional activation of HIV by Mycobacterium tuberculosis in human monocytes.
复制标题
人类单核细胞中结核分枝杆菌对 HIV 的转录激活。
DOI：
10.1046/j.1365-2249.1999.00952.x
复制
发表时间：
1999
影响因子：
4.6
通讯作者：
Salvekar,A
中科院分区：
文献类型：
--
作者：
Toossi,Z;Xia,L;Wu,M;Salvekar,A
Recently it has been shown that infection withMycobacterium tuberculosisincreases the replication of HIV in mononuclear cells. The objective of this study was to investigate the mechanism(s) of up-regulation of HIV in primary human monocytes. Monocytes from healthy subjects were infected with HIVin vitroand then cultured with purified protein derivative (PPD) ofM. tuberculosis. Culture supernatants were assessed for HIV p24 and cytokines. HIV expression was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR). PPD induced HIV-infected monocytes to increased expression of HIV RNA and production of HIV p24. This effect correlated with production of tumour necrosis factor-alpha (TNF-α) in monocyte cultures. However, neutralizing antibody to TNF-α only partly abrogated the PPD-induced HIV p24 in these cultures. Also, PPD and culture filtrate ofM. tuberculosisinduced HIV mRNA expression. Further, using an adenovirus infection system containing an HIV long-terminal repeat (LTR) reporter plasmid, we showed thatM. tuberculosisand its PPD induced HIV LTR. Therefore, the effect ofM. tuberculosisand its PPD on HIV replication in monocytes is primarily one of transcriptional activation.
