Complex developmental regulation of the Drosophila affinidisjuncta alcohol dehydrogenase gene in Drosophila melanogaster.
Complex developmental regulation of the Drosophila affinidisjuncta alcohol dehydrogenase gene in Drosophila melanogaster.
复制标题
黑腹果蝇中 Drosophila affinidisjuncta 乙醇脱氢酶基因的复杂发育调控。
DOI：
10.1016/0012-1606(88)90059-0
复制
发表时间：
1988
影响因子：
2.7
通讯作者：
Dickinson,WJ
中科院分区：
文献类型：
--
作者：
Brennan,MD;Dickinson,WJ
在发育过程中，果蝇和果蝇的乙醇脱氢酶基因在发育过程中发生了突变。affinidisjuncta以相似但不同的组织和阶段特异性模式表达。这两个基因的转录物都来自两个启动子（远端和近端），它们也显示出组织和阶段特异性。我们利用P元件介导的转化方法将D. affinidisjuncta Adh基因导入D.黑腹果蝇我们表明，D。affinidisjuncta Adh基因在两个物种中以相当的总体水平表达，并且该基因的组织特异性和阶段特异性表达（包括启动子利用）在供体和宿主物种中相似。然而，在某些细节上，D. affinidisjunctagene在D. melanogaster类似的宿主模式，一个新的组织特异性表达表型显示的转化体。在一般情况下，我们的研究结果表明，有很强的保守ofcis-和trans-acting调节因子，因为这两个物种的分歧，但这种保守并不完美。
During development, the alcohol dehydrogenase genes ofDrosophila melanogasterandD. affinidisjunctaare expressed in similar, yet distinct, tissue- and stage-specific patterns. Transcripts from both of these genes arise from two promoters (distal and proximal) that also display tissue and stage specificity. We used P-element-mediated transformation to introduce theD. affinidisjuncta Adhgene into the germ line ofD. melanogaster. We show that theD. affinidisjuncta Adhgene is expressed at comparable overall levels in both species and that the tissue- and stage-specific expression for this gene (including promoter utilization) is similar in the donor and the host species. However, in some details, the expression of theD. affinidisjunctagene inD. melanogasterresembles the host pattern, and one novel tissue-specific expression phenotype is displayed by transformants. In general, our results suggest that there has been strong conservation ofcis- andtrans-acting regulatory factors since the divergence of the two species but that this conservation has not been perfect.