Comparative studies on the roles of mediator molecules in expression of the suppressor activity of Mycobacterium avium complex-induced immunosuppressive macrophages against T cell and B cell mitogenic responses
Comparative studies on the roles of mediator molecules in expression of the suppressor activity of Mycobacterium avium complex-induced immunosuppressive macrophages against T cell and B cell mitogenic responses
复制标题
DOI：
10.1111/j.1365-2249.2006.03016.x
复制
发表时间：
2006-03-01
影响因子：
4.6
通讯作者：
Tomioka, H
中科院分区：
文献类型：
--
作者：
Cai, S;Shimizu, T;Tomioka, H
鸟分枝杆菌复合体诱导的免疫抑制巨噬细胞（MAC-M Phi s）表现出抑制蛋白A诱导的T细胞有丝分裂（T细胞Con A有丝分裂）的活性。我们研究了MAC-M - phi介导的脂多糖诱导的B细胞有丝分裂（B细胞LPS有丝分裂）的抑制情况，发现如下。首先，尽管n - g -单甲基- l-精氨酸和羧基- ptio有效地阻断了MAC-M Phi对T细胞Con A有丝分裂的抑制活性，但这些no还原剂对MAC-M Phi对B细胞LPS有丝分裂的抑制作用仅受微弱影响。其次，B细胞LPS有丝分裂发生明显比T细胞Con A有丝分裂发生更容易受到MAC-M phi衍生的活性氧中间体的影响。第三，与T细胞Con A相比，B细胞LPS有丝分裂发生对其他MAC-M phi来源的抑制介质（包括游离脂肪酸、tgf - β和前列腺素E-2）的抑制作用不太敏感。第四，只有在T细胞Con A有丝分裂发生的情况下，MAC-M Phi的抑制活性强烈依赖于B7-1样分子介导的细胞与靶细胞的接触。因此，MAC-M - Phi s对T细胞和B细胞有丝分裂的抑制方式存在显著差异。
Mycobacterium avium complex-induced immunosuppressive macrophages (MAC-M Phi s) exhibit suppressor activity against concanavalin A-induced T cell mitogenesis (T cell Con A mitogenesis). We examined the profiles of the MAC-M Phi-mediated suppression of lipopolysaccharide-induced B cell mitogenesis (B cell LPS mitogenesis) and found the following. First, although N-G-monomethyl-L-arginine and carboxy-PTIO effectively blocked the MAC-M Phi's suppressor activity against T cell Con A mitogenesis, MAC-M Phi's action against B cell LPS mitogenesis was only weakly affected by these NO-reducing agents. Second, B cell LPS mitogenesis was remarkably more susceptible to MAC-M Phi-derived reactive oxygen intermediates than T cell Con A mitogenesis. Third, B cell LPS mitogenesis was less susceptible to the inhibitory effects of the other MAC-M Phi-derived suppressor mediators, including free fatty acids, TGF-beta and prostaglandin E-2, than T cell Con A mitogenesis. Fourth, MAC-M Phi's suppressor activity was strongly dependent on B7-1 like molecule-mediated cell contact with target cells only in the case of T cell Con A mitogenesis. Therefore, there are significant differences in the modes of suppressor action of MAC-M Phi s against T cell and B cell mitogenesis.