Aciduric microbial taxa including Scardovia wiggsiae and Bifidobacterium spp. in caries and caries free subjects
Aciduric microbial taxa including Scardovia wiggsiae and Bifidobacterium spp. in caries and caries free subjects
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DOI：
10.1016/j.anaerobe.2015.04.011
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发表时间：
2015-10-01
期刊：
影响因子：
2.3
通讯作者：
Conrads, Georg
中科院分区：
文献类型：
--
作者：
Henne, Karsten;Rheinberg, Anke;Conrads, Georg
放线菌是龋病的潜在致病菌，与变形链球菌、乳酸杆菌等共同成为龋病的指示菌。本文分析了产酸性尿酸菌、牙双歧杆菌和产酸性尿酸菌的作用和诊断预测价值。非龋蚀组（n = 20）和龋蚀组（n = 6）与对照组（n = 30）生物膜中的变形杆菌、乳酸杆菌和双歧杆菌。对于双歧杆菌属和对于B.设计了新的引物。通过实时荧光定量PCR和DNA测序证实，我们发现S. wiggsiae在龋损中的阳性率高于对照组（40%）。然而，在对照组中，我们发现了三个具有最高绝对和相对S的个体。wiggsiae数测试S。变形链球菌与沙门氏菌感染率相同。wiggsiae在龋病组中的检出率为61.5%，而在对照组中的检出率仅为10%。B。在健康斑块中未发现牙本质，但在30.8%的临床病例中发现牙本质，其中在空洞病变中最多。双歧杆菌属特异性PCR的区分能力较低，因为更多的对照样品为阳性。我们计算了相对丰度和应用接收器操作特性分析。当约束集为“乳酸杆菌相对丰度>= 0.02%”和“两种相对丰度均>0.007%的耐酸菌种"时，发现特异性（93%和87%）和敏感性（100%和88%）的最高结果。几个耐酸类群的组合测量可能是有用的，以揭示龋齿活动，甚至预测龋齿的进展。(C)2015爱思唯尔有限公司版权所有。
Actinobacteria came into focus of being potential caries-associated pathogens and could, together with the established Streptococcus mutans and lactobacilli thus function as caries indicator species. Here we analyzed the role and diagnostic predictive value of the acidogenic aciduric species Scardovia wiggsiae and Bifidobacterium dentium together with S. mutans, lactobacilli and bifidobacteria in biofilm of non-cavitated (n = 20) and cavitated (n = 6) caries lesions versus controls (n = 30). For the genus Bifidobacterium and for B. dentium new sets of primers were designed. Based on real-time quantitative PCR and confirmed by DNA sequencing we found a higher prevalence (61.5%) of S. wiggsiae in caries lesions than in controls (40%). However, among the controls we found three individuals with both the highest absolute and relative S. wiggsiae numbers. Testing for S. mutans revealed the same prevalence as S. wiggsiae in caries lesions (61.5%) but in controls its prevalence was only 10%. B. dentium was never found in healthy plaque but in 30.8% of clinical cases, with the highest numbers in cavitated lesions. The Bifidobacterium-genus specific PCR had less discriminative power as more control samples were positive. We calculated the relative abundances and applied receiver operating characteristic analyses. The top results of specificity (93% and 87%) and sensitivity (100% and 88%) were found when the constraint set was "Lactobacillus relative abundance >= 0.02%" and "two aciduric species with a relative abundance of each >0.007%". Combinatory measurement of several aciduric taxa may be useful to reveal caries activity or even to predict caries progression. (C) 2015 Elsevier Ltd. All rights reserved.