Towards the complete small RNome of Acinetobacter baumannii.
Towards the complete small RNome of Acinetobacter baumannii.
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走向鲍曼不动杆菌的完整小RNome。
DOI：
10.1099/mgen.0.000045
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发表时间：
2016
影响因子：
3.9
通讯作者：
Shaw,LindseyN
中科院分区：
文献类型：
--
作者：
Weiss,Andy;Broach,WilliamH;Lee,MackenzieC;Shaw,LindseyN
近年来，革兰氏阴性菌鲍曼不动杆菌（Acinetobacter baumannii）因其对抗菌药物迅速产生耐药性的能力而引起了人们的广泛关注。与此同时，新的超强毒株似乎正在流行。尽管A.虽然已经很好地描述了鲍曼不动杆菌分离株，但这些研究主要集中在蛋白质因子上。目前，只有有限的出版物研究了小调控RNA（sRNA）转录本的存在和作用。在此，我们进行了这样的分析，描述了78 A的基于RNA-seq的鉴定。在AB 5075背景中的鲍曼不动RNA。我们将这些元素与之前确定的六个元素一起包含在新的基因组注释文件中，该文件将作为研究该生物体中调控事件的工具。我们的工作揭示了sRNA显示出高表达，占20个最强表达基因的> 50%。通过保守性分析，我们鉴定了六类相似的sRNA，其中一类与噬菌体中发现的C4反义RNA调节性RNA特别丰富且同源。这些元件似乎是以与噬菌体C4分子类似的方式从较大的转录物加工而来的，并且由两个与它们强反义的另外的sRNA控制。总的来说，这项研究提供了一个详细的视图的sRNA含量的A。鲍曼不动杆菌，揭示了这些元件之间的序列和结构保守性，并提供了对这些未充分研究的调节分子的潜在进化和作用的新见解。
In recent years, the Gram-negative bacteriumAcinetobacter baumanniihas garnered considerable attention for its unprecedented capacity to rapidly develop resistance to antibacterial therapeutics. This is coupled with the seemingly epidemic emergence of new hyper-virulent strains. Although strain-specific differences forA. baumanniiisolates have been well described, these studies have primarily focused on proteinaceous factors. At present, only limited publications have investigated the presence and role of small regulatory RNA (sRNA) transcripts. Herein, we perform such an analysis, describing the RNA-seq-based identification of 78A. baumanniisRNAs in the AB5075 background. Together with six previously identified elements, we include each of these in a new genome annotation file, which will serve as a tool to investigate regulatory events in this organism. Our work reveals that the sRNAs display high expression, accounting for >50 % of the 20 most strongly expressed genes. Through conservation analysis we identified six classes of similar sRNAs, with one found to be particularly abundant and homologous to regulatory, C4 antisense RNAs found in bacteriophages. These elements appear to be processed from larger transcripts in an analogous manner to the phage C4 molecule and are putatively controlled by two further sRNAs that are strongly antisense to them. Collectively, this study offers a detailed view of the sRNA content ofA. baumannii, exposing sequence and structural conservation amongst these elements, and provides novel insight into the potential evolution, and role, of these understudied regulatory molecules.