Regulation of hepatic glycogenolysis and vasoconstriction during antigen-induced anaphylaxis.
Regulation of hepatic glycogenolysis and vasoconstriction during antigen-induced anaphylaxis.
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抗原诱导过敏反应期间肝糖原分解和血管收缩的调节。
DOI：
10.1152/ajpgi.1992.262.5.g868
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Fisher,RA
中科院分区：
文献类型：
--
作者：
Hines,KL;Fisher,RA
本文观察了致敏抗原(卵清蛋白)对致敏大鼠及致敏大鼠离体肝的各项生理及肝脏参数的影响。致敏但不致敏的大鼠门静脉循环中注射卵清蛋白(500微克)后，体动脉压迅速持续下降，表现为抗原诱导的过敏反应，肝脏门脉压力和血糖浓度显著升高。这些抗原介导的改变类似于给大鼠注射血小板激活因子(PAF)(0.1微克/公斤)，并被特异性PAF受体拮抗剂WEB 2086(250微克/公斤)显著抑制。向致敏大鼠肝脏灌流卵清蛋白(3.8微克/毫升)可显著增加肝脏葡萄糖输出量和门静脉压力，降低氧耗量，与PAF(0.28 NM)灌流大鼠肝脏的反应相同。这些肝脏对卵清蛋白的反应是抗原特异性的，在非致敏大鼠肝脏灌流中没有观察到。WEB 2086(500 NM)可显著抑制肝脏对卵白蛋白的血流动力学和糖原分解反应，但效果不如对PAF的类似反应。将消炎痛(2.8微米)和去甲二氢愈创木酸(1微米)与WEB 2086(500毫微米)共注入肝脏可进一步抑制对卵白蛋白的血流动力学反应，但不影响糖原分解反应。向致敏的大鼠肝脏灌流一氧化氮(34微米)可阻止对卵清蛋白和PAF的血流动力学和糖原分解反应。这些观察提供了在抗原诱导的过敏反应中刺激肝糖原分解和血管收缩的证据，并表明这些反应部分是由PAF介导的。
Effects of sensitizing antigen (ovalbumin) on various physiological and hepatic parameters were investigated in sensitized rats and isolated perfused livers derived from sensitized rats. Administration of ovalbumin (500 micrograms) to the portal venous circulation of sensitized but not nonsensitized rats resulted in a rapid and sustained decrease in systemic arterial pressure, characteristic of antigen-induced anaphylaxis, and pronounced increases in hepatic portal pressure and blood glucose concentration. These antigen-mediated alterations were similar to those observed in response to platelet-activating factor (PAF) (0.1 micrograms/kg) administration to rats and were inhibited significantly by specific PAF receptor antagonist WEB 2086 (250 micrograms/kg). Infusion of ovalbumin (3.8 micrograms/ml) into isolated perfused livers derived from sensitized rats resulted in significant increases in hepatic glucose output and portal pressure and decreases in oxygen consumption, as observed in response to PAF (0.28 nM) infusion into perfused livers. These hepatic responses to ovalbumin were antigen specific and were not observed in nonsensitized rat perfused livers. Hemodynamic and glycogenolytic responses to ovalbumin in perfused livers were inhibited significantly but less effectively than similar responses to PAF by infusion of WEB 2086 (500 nM) into livers. Coinfusion of indomethacin (2.8 microM) and nordihydroguariatic acid (1 microM) with WEB 2086 (500 nM) into perfused livers inhibited further hemodynamic but not glycogenolytic responses to ovalbumin. Infusion of nitric oxide (34 microM) into sensitized rat perfused livers prevented the hemodynamic and glycogenolytic responses to both ovalbumin and PAF. These observations provide evidence that hepatic glycogenolysis and vasoconstriction are stimulated during antigen-induced anaphylaxis and suggest that these responses are mediated in part by PAF.