Development of a microfluidic platform with an optical imaging microarray capable of attomolar target DNA detection
Development of a microfluidic platform with an optical imaging microarray capable of attomolar target DNA detection
复制标题
DOI：
10.1021/ac050503t
复制
发表时间：
2005-09-01
影响因子：
7.4
通讯作者：
Walt, DR
中科院分区：
文献类型：
--
作者：
Bowden, M;Song, LN;Walt, DR
在本文中，利用光纤微阵列上的荧光检测技术，在微流控歧管中进行DNA杂交。该微流控装置在单一平台上集成了光学、样品传输和流体互连。研制了一种含有寡核苷酸标记微球的高密度光学成像光纤阵列。用50亩L的目标DNA样品，以1亩L/分钟的流速，在低至上午10点的浓度下，反应时间小于15分钟，观察到DNA杂交。快速的响应时间加上低采样体积以及高密度光纤微阵列格式的使用，使得该方法具有很大的优势。本文描述了微流控平台与成像光纤阵列的初步开发、优化和集成。
In this paper, DNA hybridization in a microfluidic manifold is performed using fluorescence detection on a fiber-optic microarray. The microfluidic device integrates optics, sample transport, and fluidic interconnects on a single platform. A high-density optical imaging fiber array containing oligonucleotide-labeled microspheres was developed. DNA hybridization was observed at concentrations as low as 10 aM with response times of less than 15 min at a flow rate of 1 mu L/min using 50 mu L of target DNA samples. The fast response times coupled with the low sample volumes and the use of a high-density, fiber-optic microarray format make this method highly advantageous. This paper describes the initial development, optimization, and integration of the microfluidic platform with imaging fiber arrays.