Effects of culture medium salinity on the hydrocarbon extractability, growth and morphology of Botryococcus braunii
Effects of culture medium salinity on the hydrocarbon extractability, growth and morphology of Botryococcus braunii
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培养基盐度对布氏葡萄球菌碳氢化合物可萃取性、生长和形态的影响
DOI：
10.1016/j.biombioe.2016.05.007
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发表时间：
2016
影响因子：
6
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Furuhashi;K.;Hasegawa;F.;Saga;K.;Kudou;S.;Okada;S.;Kaizu;Y.;Imou;K.
本研究研究了培养基盐度对昭和葡萄球菌（botryococcus brauniistrain Showa）烃类提取能力、生长和形态的影响。在所有测试的盐度下，在7天内发生的第一次或第二次细胞分裂期间，碳氢化合物的采收率都有所增加。在改良的Chu13培养基中，藻类的生长与在0.3%海水盐度（0.3% sm）培养基中，将海水（3.6%，盐度为36 g L−1）稀释后的藻类生长无显著差异。相比之下，在0.3%的海水盐度下培养提高了碳氢化合物的可提取性，缩短了碳氢化合物提取所需的时间。这种变化被认为是由于多糖产量的减少，多糖阻止非极性溶剂与碳氢化合物接触。此外，在0.3%盐度的海水中培养时，藻类的菌落大小和浮力都有所增加。我们的实验结果表明，培养b。在适当盐度的培养基中，可以减少从收获到提取碳氢化合物的投入能量和成本，而不会减少生物质产量。
Here, we investigated the effects of culture medium salinity on the hydrocarbon extractability, growth and morphology ofBotryococcus brauniistrain Showa. At all tested salinities, the hydrocarbon recovery rate increased during the first or second cell division, which occurred within 7 days. Algal growth in modified Chu13 medium did not differ significantly from that in medium with 0.3% seawater salinity (0.3%SM) to which seawater (3.6%, salinity was 36 g L−1) was diluted. In contrast, culturing in 0.3% seawater salinity improved hydrocarbon extractability and shortened the time required for hydrocarbon extraction. This change supposed to be due to the decrease in the production of polysaccharides which prevent non-polar solvent from contacting hydrocarbons. Additionally, there was an increase in the colony sizes and flotation of the alga when cultured in 0.3% seawater salinity. The results of our experiments suggest that culturingB. brauniiin a medium with the appropriate salinity can reduce the input energy and the costs incurred from harvest to hydrocarbon extraction without decreasing biomass production.