Abnormal vibrissa-related behavior and loss of barrel field inhibitory neurons in 5xFAD transgenics.
Abnormal vibrissa-related behavior and loss of barrel field inhibitory neurons in 5xFAD transgenics.
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DOI：
10.1111/gbb.12133
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
McDonald MP
中科院分区：
文献类型：
--
作者：
Flanigan TJ;Xue Y;Kishan Rao S;Dhanushkodi A;McDonald MP
最近的一项研究报告了阿尔茨海默病的5xFAD转基因小鼠模型中较低的焦虑，这是通过减少高架十字迷宫开放臂上的时间来测量的。这一点很重要，因为实验动物的所有行为都必须根据基础焦虑和对新环境的反应来解释。我们在5xFAD转基因动物中进行了全面的焦虑测试，并复制了十字迷宫表型。然而，我们发现，它并没有反映减少焦虑，而是异常回避的封闭武器的一部分，转基因和会话内习惯于封闭武器的一部分，野生型控制。我们注意到，5xFAD转基因小鼠没有这种背景品系小鼠典型的胡须-毛发行为。这暗示着异常的社会行为，我们怀疑这可能与他们避免在十字迷宫中闭合的手臂有关。事实上，转基因小鼠表现出过度的笼中社会行为和受损的社会识别，并不允许野生型小鼠在成对饲养时理发。当他们的胡须被剪掉时，5xFAD转基因动物不再避开加号迷宫上的闭合臂。小清蛋白（PV）染色的检查显示在5xFAD小鼠的桶内视野中PV+抑制性中间神经元减少28.9%，并且在IV层和V层中PV+纤维的损失。触须抑制的这种损失表明可能导致转基因动物在十字迷宫中避免闭合臂的puperminaravoidable过度刺激。
A recent study reported lower anxiety in the 5xFAD transgenic mouse model of Alzheimer's disease, as measured by reduced time on the open arms of an elevated plus maze. This is important because all behaviors in experimental animals must be interpreted in light of basal anxiety and response to novel environments. We conducted a comprehensive anxiety battery in the 5xFAD transgenics and replicated the plus-maze phenotype. However, we found that it did not reflect reduced anxiety, but rather abnormal avoidance of the closed arms on the part of transgenics and within-session habituation to the closed arms on the part of wild-type controls. We noticed that the 5xFAD transgenics did not engage in the whisker-barbering behavior typical of mice of this background strain. This is suggestive of abnormal social behavior, and we suspected it might be related to their avoidance of the closed arms on the plus maze. Indeed, transgenic mice exhibited excessive home-cage social behavior and impaired social recognition, and did not permit barbering by wild-type mice when pair-housed. When their whiskers were snipped the 5xFAD transgenics no longer avoided the closed arms on the plus maze. Examination of parvalbumin (PV) staining showed a 28.9% reduction in PV+ inhibitory interneurons in the in barrel fields of 5xFAD mice, and loss of PV+ fibers in layers IV and V. This loss of vibrissal inhibition suggests a putatively aversive overstimulation that may be responsible for the transgenics’ avoidance of the closed arms in the plus maze.
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发表时间：
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期刊：
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