Nasal swab bacteriology by PCR during the first 24-months of life: A prospective birth cohort study
Nasal swab bacteriology by PCR during the first 24-months of life: A prospective birth cohort study
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DOI：
10.1002/ppul.24231
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发表时间：
2019-03-01
影响因子：
3.1
通讯作者：
Grimwood, Keith
中科院分区：
文献类型：
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作者：
Palmu, Arto A.;Ware, Robert S.;Grimwood, Keith
背景大多数儿童呼吸道细菌携带研究是基于横断面样本或纵向研究，采样点不频繁。儿童传染病前瞻性观察性研究出生队列研究集中评估了生命最初2年内以社区为基础的呼吸道病毒和细菌流行病学。在这里，我们报告的细菌学findings.MethodsPregnancy妇女在布里斯班，澳大利亚被招募2010年9月和2012年10月之间，他们的健康新生儿进行了跟踪的第一个2年的生活。父母为研究儿童保留每日症状日记，每周收集前鼻拭子，并在他们的孩子符合预定义的疾病标准时完成疾病负担日记。标本进行了测试，呼吸道细菌的实时聚合酶链反应（PCR）检测和那些含有人类基因组DNA，被认为是高品质的，进行了analysed.ResultsAlong 8100高品质的鼻拭子标本158名儿童进行了分析。肺炎链球菌、卡他莫拉菌和流感嗜血杆菌的检出率分别为42.4%、38.9%和14.8%。常见伴随细菌检测。与此相反，百日咳杆菌，B。副百日咳、肺炎支原体、肺炎衣原体和Simkania negevensis很少被鉴定。三种主要细菌的患病率随着年龄的增长和在冬季和春季月份更高。similarity和儿童保育出勤率的其他风险因素identified.ConclusionsWe证实了频繁的鼻拭子的可行性，由父母研究细菌定植。PCR检测到的主要呼吸道细菌与预期的高频率，但非典型细菌很少发现在这个队列。
BackgroundMost respiratory bacterial carriage studies in children are based on cross-sectional samples or longitudinal studies with infrequent sampling points. The prospective Observational Research in Childhood Infectious Diseases birth cohort study intensively evaluated the community-based epidemiology of respiratory viruses and bacteria during the first 2-years of life. Here we report the bacteriologic findings.MethodsPregnant women in Brisbane, Australia were recruited between September 2010 and October 2012, and their healthy newborn children were followed for the first 2-years of life. Parents kept a daily symptom diary for the study child, collected a weekly anterior nose swab and completed an illness burden diary when their child met pre-defined illness criteria. Specimens were tested for respiratory bacteria by real-time polymerase chain reaction (PCR) assays and those containing human genomic DNA, deemed as high-quality, were analyzed.ResultsAltogether 8100 high-quality nasal swab specimens from 158 enrolled children were analyzed. Streptococcus pneumoniae, Moraxella catarrhalis, and Haemophilus influenzae were detected in 42.4%, 38.9%, and 14.8% of these samples, respectively. Concomitant detection of bacteria was common. In contrast, Bordetella pertussis, B. parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Simkania negevensis were rarely identified. The prevalence of the three major bacteria was higher with increasing age and in the winter and spring months. Siblings and childcare attendance were the other risk factors identified.ConclusionsWe confirmed the feasibility of frequent nasal swabbing by parents for studying bacterial colonization. PCR detected the major respiratory tract bacteria with expected high frequencies, but atypical bacteria were found rarely in this cohort.