Telomerase catalytic subunit homologs from fission yeast and human
Telomerase catalytic subunit homologs from fission yeast and human
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DOI：
10.1126/science.277.5328.955
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发表时间：
1997-08-15
期刊：
影响因子：
56.9
通讯作者：
Cech, TR
中科院分区：
文献类型：
--
作者：
Nakamura, TM;Morin, GB;Cech, TR
端粒酶的催化蛋白亚基来自纤毛虫atuculatus和酵母酿酒酵母含有逆转录酶基序。本文鉴定了分裂酵母、裂糖酵母和人类的同源基因。S. pombe基因的破坏导致端粒缩短和衰老，人类基因mRNA的表达与细胞系端粒酶活性相关。序列比较将端粒酶蛋白置于逆转录酶家族中，但揭示了将它们与逆转录病毒和反转录转座子亲戚区分开来的特征。因此，提出的端粒酶催化亚基在系统发育上是保守的，代表了逆转录酶进化的一个深分支。
Catalytic protein subunits of telomerase from the ciliate Euplotes aediculatus and the yeast Saccharomyces cerevisiae contain reverse transcriptase motifs. Here the homologous genes from the fission yeast Schizosaccharomyces pombe and human are identified. Disruption of the S. pombe gene resulted in telomere shortening and senescence, and expression of mRNA from the human gene correlated with telomerase activity in cell lines. Sequence comparisons placed the telomerase proteins in the reverse transcriptase family but revealed hallmarks that distinguish them from retroviral and retrotransposon relatives. Thus, the proposed telomerase catalytic subunits are phylogenetically conserved and represent a deep branch in the evolution of reverse transcriptases.