Microbiota profile in feces of breast- and formula-fed newborns by using fluorescence in situ hybridization (FISH)
Microbiota profile in feces of breast- and formula-fed newborns by using fluorescence in situ hybridization (FISH)
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DOI：
10.1016/j.anaerobe.2011.03.009
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发表时间：
2011-12-01
期刊：
影响因子：
2.3
通讯作者：
Welling, Gjalt W.
中科院分区：
文献类型：
--
作者：
Bezirtzoglou, Eugenia;Tsiotsias, Arsenis;Welling, Gjalt W.
肠道的发育受到不同相互作用机制的控制和调节，例如遗传禀赋、内在生物调节功能、环境影响以及最后但并非最不重要的饮食影响。与其他内源性和外源性因素一起考虑，喂养类型可能会极大地干扰肠道微生物群的调节。在过去的几年中，分子方法提供了一个补充的经典文化为基础的知识。FISH已被应用于阴道分娩新生儿微生物群的分子评估。11个探针/探针组合的特定群体的粪便细菌被用来确定细菌组成的新生儿的粪便样本下，不同类型的喂养。母乳喂养的婴儿的粪便微生物群中双歧杆菌细胞的数量比配方奶粉喂养的婴儿增加了两倍多。配方饲料喂养后，异形菌数量显著增加，类杆菌中双歧杆菌数量下降，随后数量增加。此外，配方奶粉喂养的婴儿微生物群更加多样化。(C)2011爱思唯尔有限公司保留所有权利。
The development of the gut is controlled and modulated by different interacting mechanisms such as, genetic endowment, intrinsic biological regulatory functions, environment influences and last but no least, the diet influence. Considered together with other endogenous and exogenous factors the type of feeding may interfere greatly in the regulation of the intestinal microbiota. During the last years molecular methods offer a complementarity to the classic culture-based knowledge. FISH has been applied for molecular evaluation of the microbiota in newborns delivered by vaginal delivery. Eleven probes/probe combinations for specific groups of faecal bacteria were used to determine the bacterial composition in faecal samples of newborns infants under different types of feeding. Breast-fed infants harbor a fecal microbiota by more than two times increased in numbers of Bifidobacterium cells when compared to formula-fed infants. After formula-feeding, Atopobium was found in significant counts and the numbers of Bifidobacterium dropped followed by increasing numbers in Bacteroides population. Moreover, under formula feeding the infants microbiota was more diverse. (C) 2011 Elsevier Ltd. All rights reserved.