Complete genomic DNA sequence of the East Asian spotted fever disease agent Rickettsia japonica.
Complete genomic DNA sequence of the East Asian spotted fever disease agent Rickettsia japonica.
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DOI：
10.1371/journal.pone.0071861
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shirai M
中科院分区：
文献类型：
--
作者：
Matsutani M;Ogawa M;Takaoka N;Hanaoka N;Toh H;Yamashita A;Oshima K;Hirakawa H;Kuhara S;Suzuki H;Hattori M;Kishimoto T;Ando S;Azuma Y;Shirai M
日本立克次体是一种专性细胞内α-变形菌，可引起蜱传日本斑疹热，该斑疹热已蔓延至整个东亚。测定了该菌的全基因组DNA序列。japonica型菌株YH（VR-1363），全长1，283，087 bp，编码971个蛋白质。比较了红毛菊的基因组DNA序列。日本立克次体与其他立克次体在公共数据库中的比较显示，在一个非常窄的立克次体物种范围内有2个区域（4，323和216 bp）是保守的，并且较短的一个被插入并破坏了预先存在的开放阅读框架（ORF）。目前尚不清楚这些DNA序列是如何在R.日本立克次体基因组中，它可能是一个有用的签名，用于诊断立克次体物种。立克次体基因组含有立克次体特异性回文元件（RPE），而不是种特异性插入的DNA序列，这些回文元件也能够定位于预先存在的ORF中。对涉及RPE的蛋白质和DNA序列的精确比对表明，当一个基因含有插入的DNA序列时，每个立克次体直系同源物在同一位点携带插入的DNA序列。序列ATGAC显示为高频率的，因此在某些RPE（RPE-4、RPE-6和RPE-7）中是特征性的。这一发现意味着RPE-4、RPE-6和RPE-7来源于共同的插入DNA序列。
Rickettsia japonica is an obligate intracellular alphaproteobacteria that causes tick-borne Japanese spotted fever, which has spread throughout East Asia. We determined the complete genomic DNA sequence of R. japonica type strain YH (VR-1363), which consists of 1,283,087 base pairs (bp) and 971 protein-coding genes. Comparison of the genomic DNA sequence of R. japonica with other rickettsiae in the public databases showed that 2 regions (4,323 and 216 bp) were conserved in a very narrow range of Rickettsia species, and the shorter one was inserted in, and disrupted, a preexisting open reading frame (ORF). While it is unknown how the DNA sequences were acquired in R. japonica genomes, it may be a useful signature for the diagnosis of Rickettsia species. Instead of the species-specific inserted DNA sequences, rickettsial genomes contain Rickettsia-specific palindromic elements (RPEs), which are also capable of locating in preexisting ORFs. Precise alignments of protein and DNA sequences involving RPEs showed that when a gene contains an inserted DNA sequence, each rickettsial ortholog carried an inserted DNA sequence at the same locus. The sequence, ATGAC, was shown to be highly frequent and thus characteristic in certain RPEs (RPE-4, RPE-6, and RPE-7). This finding implies that RPE-4, RPE-6, and RPE-7 were derived from a common inserted DNA sequence.
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影响因子：
11.8
作者：
Chung MH;Lee SH;Kim MJ;Lee JH;Kim ES;Lee JS;Kim MK;Park MY;Kang JS
通讯作者：
Kang JS
影响因子：
4.5
作者：
Blanc, Guillaume;Ogata, Hiroyuki;Robert, Catherine;Audic, Stephane;Suhre, Karsten;Vestris, Guy;Claverie, Jean-Michel;Raoult, Didier
通讯作者：
Raoult, Didier
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
DOI：
10.1196/annals.1374.007
发表时间：
2006-01-01
期刊：
CENTURY OF RICKETTSIOLOGY: EMERGING, REEMERGING RICKETTSIOSES, MOLECULAR DIAGNOSTICS, AND EMERGING VETERINARY RICKETTSIOSES
影响因子：
--
作者：
Mahara, Fumihiko
通讯作者：
Mahara, Fumihiko
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.