Abnormal Small-Intestinal Endocrine Cells in Patients with Irritable Bowel Syndrome
Abnormal Small-Intestinal Endocrine Cells in Patients with Irritable Bowel Syndrome
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DOI：
10.1007/s10620-010-1169-6
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发表时间：
2010-12-01
影响因子：
3.1
通讯作者：
Hausken, T.
中科院分区：
文献类型：
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作者：
El-Salhy, Magdy;Vaali, K.;Hausken, T.
肠易激综合征（IBS）患者有胃肠运动障碍的报道。胃肠道激素在调节胃肠运动中起着重要作用。探讨肠易激综合征患者小肠内分泌细胞可能的异常。该研究包括41名符合罗马标准III的肠易激综合征患者和42名健康对照者。胃镜检查时对患者和对照组进行十二指肠活检。活检组织采用亲和素-生物素复合物法对分泌素、CCK、GIP、生长抑素和血清素细胞进行免疫染色。通过计算机图像分析定量细胞密度。肠易激综合征患者的分泌素和cck免疫反应细胞密度显著降低。分泌素和CCK细胞的减少仅发生在ibs -腹泻患者中，而不发生在ibs -便秘亚型中。肠易激综合征患者十二指肠GIP和生长抑素细胞密度均降低。IBS患者各亚型之间在分泌素、CCK、GIP或生长抑素细胞密度方面没有统计学差异。肠易激综合征患者血清素细胞密度不受影响。ibs -腹泻患者分泌素和CCK细胞的低密度可能导致胰腺功能不全和胆囊排空不足，因为这些激素刺激胰腺碳酸氢盐和酶的分泌，CCK也刺激胆囊收缩。IBS患者体内分泌素、GIP和生长抑素细胞密度低可能导致胃酸分泌增多，因为分泌素、GIP和生长抑素抑制胃酸分泌。
General disturbances in gastrointestinal motility have been reported in patients with irritable bowel syndrome (IBS). The gastrointestinal tract hormones play an important role in regulating gastrointestinal motility.To investigate a possible abnormality in the small intestinal endocrine cells of IBS patients.Included in the study were 41 patients with irritable bowel syndrome according to Rome Criteria III and 42 healthy controls. Duodenal biopsies were obtained from both patients and controls during gastroscopy. The biopsies were immunostained by avidin-biotin-complex method for secretin, CCK, GIP, somatostatin, and serotonin cells. The cell densities were quantified by computerized image analysis.The density of secretin- and CCK-immunoreactive cells in patients with IBS was significantly reduced. The reduction in secretin and CCK cells occurred only in IBS-diarrhea patients, but not in IBS-constipation subtype. Both GIP and somatostatin cell densities were reduced in the duodenum of IBS patients. There was no statistical difference between the subtypes of IBS patients, regarding secretin, CCK, GIP, or somatostatin cell densities. Serotonin cell density was not affected in patients with IBS.The low densities of secretin and CCK cells in IBS-diarrhea patients may cause a functional pancreatic insufficiency as well as inadequate gall emptying, as these hormones stimulate pancreatic bicarbonate and enzyme secretion and CCK stimulates as well gall bladder contraction. Low densities of secretin, GIP, and somatostatin cells in IBS patients might result in a high secretion of gastric acid, as secretin, GIP, and somatostatin inhibit gastric acid secretion.