Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.
Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.
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DOI：
10.1073/pnas.87.24.9853
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发表时间：
1990-12
影响因子：
11.1
通讯作者：
K. Shinjo;J. Koland;Matthew J. Hart;Vikram Narasimhan;Douglas I. Johnson;T. Evans;R. Cerione
中科院分区：
文献类型：
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作者：
K. Shinjo;J. Koland;Matthew J. Hart;Vikram Narasimhan;Douglas I. Johnson;T. Evans;R. Cerione
我们已经从人胎盘文库中分离出编码低分子量GTP结合蛋白的cDNA克隆，该蛋白最初被命名为GP(也称为G25K)。这种鉴定是基于与纯化的人GP蛋白的可用多肽序列的比较以及两种高度特异的抗肽抗体的使用。该蛋白的预测氨基酸序列与低分子量GTP结合蛋白ras超家族的各种成员非常相似，包括N-、KI-和Ha-ras蛋白(30-35%相同)，Rho蛋白(约50%相同)和Rac蛋白(约70%相同)。序列同源性最高的是酿酒酵母细胞分裂周期蛋白CDC42(80%)。人类胎盘基因，我们命名为CDC42Hs，补充了酿酒酵母中的CDC42-1突变，这表明这种GTP结合蛋白是酵母蛋白的人类同源物。
We have isolated cDNA clones from a human placental library that code for a low molecular weight GTP-binding protein originally designated Gp (also called G25K). This identification is based on comparisons with the available peptide sequences for the purified human Gp protein and the use of two highly specific anti-peptide antibodies. The predicted amino acid sequence of the protein is very similar to those of various members of the ras superfamily of low molecular weight GTP-binding proteins, including the N-, Ki-, and Ha-ras proteins (30-35% identical), the rho proteins (approximately 50% identical), and the rac proteins (approximately 70% identical). The highest degree of sequence identity (80%) is found with the Saccharomyces cerevisiae cell-division-cycle protein CDC42. The human placental gene, which we designate CDC42Hs, complements the cdc42-1 mutation in S. cerevisiae, which suggests that this GTP-binding protein is the human homolog of the yeast protein.