Transcorneal Electrical Stimulation Shows Neuroprotective Effects in Retinas of Light-Exposed Rats
Transcorneal Electrical Stimulation Shows Neuroprotective Effects in Retinas of Light-Exposed Rats
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DOI：
10.1167/iovs.12-10037
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发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Gekeler, Florian
中科院分区：
文献类型：
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作者：
Schatz, Andreas;Arango-Gonzalez, Blanca;Gekeler, Florian
目的。目的观察经角膜电刺激（TES）对光暴露大鼠视网膜变性的影响。33只Sprague Dawley白化大鼠分为三组：STIM组（n = 15）接受60分钟的TES， SHAM组（n = 15）在暴露于16000勒克斯强光前2小时接受相同的假刺激；健康动物（n = 3）作为组织学对照。在基线和每周连续3周，使用适应暗和光的视网膜电图评估视网膜功能。通过对响应-亮度函数的分析，得到了Vmax（饱和度振幅）和k（达到1/2Vmax的亮度）参数。采用组织学（苏木精-伊红[HE]染色；TUNEL染色）和免疫组织化学（视紫红质染色）评价视网膜形态。光损伤后1周，STIM组Vmax高于SHAM组（组间个体内平均差异116.06 mu V, P = 0.046）。光损伤后2周，STIM组杆状反应的b波隐时间（0.01 cd.s/m(2)）低于SHAM组（组内平均个体差异5.78 ms, P = 0.023）；未发现其他显著差异。组织学分析显示，假手术中光感受器细胞死亡（TUNEL和HE），以上半视网膜最为明显。与SHAM （HE和视紫红质）相比，STIM能完全保存外核层厚度，减少光感受器细胞死亡，并保留外核段长度。这项假对照研究表明，TES可以保护Sprague Dawley大鼠的视网膜细胞免受轻度光诱导变性。这些发现可能有助于确立TES作为一种治疗人类视网膜退行性疾病的方法。（Invest Ophthalmol Vis Sci. 2012;53:5552-5561） DOI: 10.1167/iovs.12-10037
PURPOSE. To examine the effects of transcorneal electrical stimulation (TES) on retinal degeneration of light-exposed rats.METHODS. Thirty-three Sprague Dawley albino rats were divided into three groups: STIM (n = 15) received 60 minutes of TES, whereas SHAM (n = 15) received identical sham stimulation 2 hours before exposure to bright light with 16,000 lux; healthy animals (n = 3) served as controls for histology. At baseline and weekly for 3 consecutive weeks, dark-and light-adapted electroretinography was used to assess retinal function. Analysis of the response versus luminance function retrieved the parameters Vmax (saturation amplitude) and k (luminance to reach 1/2Vmax). Retinal morphology was assessed by histology (hematoxylin-eosin [HE] staining; TUNEL assay) and immunohistochemistry (rhodopsin staining).RESULTS. Vmax was higher in the STIM group compared with SHAM 1 week after light damage (mean intra-individual difference between groups 116.06 mu V; P = 0.046). The b-wave implicit time for the rod response (0.01 cd.s/m(2)) was lower in the STIM group compared with the SHAM group 2 weeks after light damage (mean intra-individual difference between groups 5.78 ms; P = 0.023); no other significant differences were found. Histological analyses showed photoreceptor cell death (TUNEL and HE) in SHAM, most pronounced in the superior hemiretina. STIM showed complete outer nuclear layer thickness preservation, reduced photoreceptor cell death, and preserved outer segment length compared with SHAM (HE and rhodopsin).CONCLUSIONS. This sham-controlled study shows that TES can protect retinal cells against mild light-induced degeneration in Sprague Dawley rats. These findings could help to establish TES as a treatment in human forms of retinal degenerative disease. (Invest Ophthalmol Vis Sci. 2012;53:5552-5561) DOI: 10.1167/iovs.12-10037