Characterization of the primary spinal afferent innervation of the mouse colon using retrograde labelling
Characterization of the primary spinal afferent innervation of the mouse colon using retrograde labelling
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DOI：
10.1046/j.1365-2982.2003.00456.x
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发表时间：
2004-02-01
影响因子：
3.5
通讯作者：
Hicks, GA
中科院分区：
文献类型：
--
作者：
Robinson, DR;McNaughton, PA;Hicks, GA
内脏疼痛是由疾病产生的最常见的疼痛形式，因此在胃肠道（GI）主诉（例如肠易激综合征）的研究中是感兴趣的，其中感知为GI疼痛的感觉信号在具有背根神经节（DRG）中的细胞体的外部传入神经元中传播。小鼠降结肠的初级脊髓传入神经支配起源于背根神经节，但这一点尚未明确。本研究结合逆行标记和免疫组织化学，以确定和表征这些神经元。在T8-L1和L 6-S1水平的DRG中发现了小型至中型逆行标记细胞体。降钙素基因相关肽（CGRP）和P2 X（3）样免疫反应性（LI）分别见于81%和32%的逆行标记细胞，20%的细胞结合了来自单叶格里芬属植物的同种凝集素IB 4。CGRP-LI和IB 4共定位于22%的逆行标记细胞中，而P2 X（3）-LI和IB 4共定位于7%的逆行标记细胞中（相对于在整个DRG群体中观察到的34%）。82%的逆行标记细胞表现出香草素受体1样免疫反应性（VR 1-LI）。这些结果表明，小鼠结肠脊髓初级传入神经元主要是含CGRP的肽能、VR 1-LI、C纤维传入。与一般DRG群体相反，存在P2 X（3）受体-LI但不结合IB 4的神经元子集。
Visceral pain is the most common form of pain produced by disease and is thus of interest in the study of gastrointestinal (GI) complaints such as irritable bowel syndrome, in which sensory signals perceived as GI pain travel in extrinsic afferent neurones with cell bodies in the dorsal root ganglia (DRG). The DRG from which the primary spinal afferent innervation of the mouse descending colon arises are not well defined. This study has combined retrograde labelling and immunohistochemistry to identify and characterize these neurones. Small to medium-sized retrogradely labelled cell bodies were found in the DRG at levels T8-L1 and L6-S1. Calcitonin gene-related peptide (CGRP)- and P2X(3)-like immunoreactivity (LI) was seen in 81 and 32%, respectively, of retrogradely labelled cells, and 20% bound the Griffonia simplicifolia-derived isolectin IB4. CGRP-LI and IB4 were co-localized in 22% of retrogradely labelled cells, whilst P2X(3)-LI and IB4 were co-localized in 7% (vs 34% seen in the whole DRG population). Eighty-two per cent of retrogradely labelled cells exhibited vanilloid receptor 1-like immunoreactivity (VR1-LI). These data suggest that mouse colonic spinal primary afferent neurones are mostly peptidergic CGRP-containing, VR1-LI, C fibre afferents. In contrast to the general DRG population, a subset of neurones exist that are P2X(3) receptor-LI but do not bind IB4.