Immobilization of Cold-Active Cellulase from Antarctic Bacterium and Its Use for Kelp Cellulose Ethanol Fermentation
Immobilization of Cold-Active Cellulase from Antarctic Bacterium and Its Use for Kelp Cellulose Ethanol Fermentation
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DOI：
10.15376/biores.10.1.1757-1772
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发表时间：
2015-01
期刊：
影响因子：
1.5
通讯作者：
Yi Bin Wang;Cong Gao;Zhou Zheng;Fang-ming Liu;J. Zang;J. Miao
中科院分区：
文献类型：
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作者：
Yi Bin Wang;Cong Gao;Zhou Zheng;Fang-ming Liu;J. Zang;J. Miao
固定化技术是解决冷活性纤维素酶工业化应用问题的有效途径。以南极嗜冷菌Pseudoalteromonas sp. NJ 64为出发菌株，对其产低温纤维素酶进行了分离、固定化，并对固定化条件进行了优化。并将其用于海带纤维素乙醇发酵，获得了纯度较高的海带纤维素乙醇。该低温纤维素酶的酶活为49.7U/mL。最佳固定化工艺条件为：海藻酸钠30 g/L，氯化钙5 g/L，戊二醛0.4%，交联时间5 h。在此条件下，酶活力回收率为51.58%。最适反应温度为40 °C，最适初始pH为9.0，回收7次后酶活力为58.37%。海带纤维素乙醇纯度较高，达到37.37%。固定化冷活性纤维素酶能有效水解海带渣中的纤维素，是纤维素生物乙醇生产的重要组成部分，对我国乙醇产业的发展具有重要意义。
Immobilization is an effective way to solve the problem associated with the application of cold-active cellulase in industrial processes. In this study, a cold-active cellulase from the Antarctic psychrophilic bacterium Pseudoalteromonas sp. NJ64 was obtained, immobilized, and analyzed for optimal immobilization conditions. Then it was used in kelp cellulose ethanol fermentation, achieving a higher purity level of kelp cellulose ethanol. The enzymatic activity of this cold-active cellulase was 49.7 U/mL. The optimal immobilization process conditions were as follows: sodium alginate, 30 g/L; calcium chloride, 5 g/L; glutaraldehyde, 0.4%; and cross-linking time, 5 h. Under these conditions, the activity recovery rate was 51.58%. The optimum reaction temperature was at 40 °C, the optimum initial pH was 9.0, and the relative enzyme activity was 58.37% after being recovered seven times. A higher purity level of kelp cellulose ethanol has reached (37.37%). Immobilized cold-active cellulase can effectively hydrolyze the cellulose of kelp residue, which is a valuable component of cellulose bio-ethanol production and will have broad implications in the development of the ethanol industry in China.