Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells.
Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells.
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DOI：
10.1084/jem.20060468
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发表时间：
2006-07-10
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CD 4 + CD 25 + Foxp 3+调节性T（T reg）细胞的缺失与过敏性、自身免疫性和免疫炎症性疾病的易感性有关。然而，人类T reg细胞的表型和功能评估受到难以区分表达CD 25的活化和调节性T细胞的阻碍。在这里，我们发现，表达CD 127，白细胞介素-7受体的α链，允许一个明确的基于流式细胞术的区分CD 127 lo T reg细胞和CD 127 hi常规T细胞在外周血和淋巴结中的CD 25 + CD 45 RO +RA−效应/记忆和CD 45 RA +RO−幼稚区室。在健康志愿者中，外周血CD 25 + CD 127 lo细胞占CD 4 + T细胞的6.35 ± 0.26%，其中2.05 ± 0.14%表达幼稚亚群标志物CD 45 RA。FoxP 3蛋白表达和CD 127 lo表型在CD 4 + CD 25+群体中高度相关。此外，效应/记忆和初始CD 25 + CD 127 lo细胞在体外表现出抑制活性，而CD 25 + CD 127 hi细胞没有。因此，CD 127的细胞表面表达允许准确估计T reg细胞数量和分离纯群体用于体外研究，并应有助于我们理解免疫性疾病中的调节异常。
Abnormalities in CD4+CD25+Foxp3+ regulatory T (T reg) cells have been implicated in susceptibility to allergic, autoimmune, and immunoinflammatory conditions. However, phenotypic and functional assessment of human T reg cells has been hampered by difficulty in distinguishing between CD25-expressing activated and regulatory T cells. Here, we show that expression of CD127, the α chain of the interleukin-7 receptor, allows an unambiguous flow cytometry–based distinction to be made between CD127lo T reg cells and CD127hi conventional T cells within the CD25+CD45RO+RA− effector/memory and CD45RA+RO− naive compartments in peripheral blood and lymph node. In healthy volunteers, peripheral blood CD25+CD127lo cells comprised 6.35 ± 0.26% of CD4+ T cells, of which 2.05 ± 0.14% expressed the naive subset marker CD45RA. Expression of FoxP3 protein and the CD127lo phenotype were highly correlated within the CD4+CD25+ population. Moreover, both effector/memory and naive CD25+CD127lo cells manifested suppressive activity in vitro, whereas CD25+CD127hi cells did not. Cell surface expression of CD127 therefore allows accurate estimation of T reg cell numbers and isolation of pure populations for in vitro studies and should contribute to our understanding of regulatory abnormalities in immunopathic diseases.