Simultaneous determination of artificial sweeteners, preservatives, caffeine, theobromine and theophylline in food and pharmaceutical preparations by ion chromatography
Simultaneous determination of artificial sweeteners, preservatives, caffeine, theobromine and theophylline in food and pharmaceutical preparations by ion chromatography
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DOI：
10.1016/s0021-9673(01)01306-1
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发表时间：
2001-12-07
影响因子：
4.1
通讯作者：
Wang, J
中科院分区：
文献类型：
--
作者：
Chen, QC;Wang, J
提出了一种同时测定人工甜味剂（糖精钠、阿斯巴甜、安赛蜜）、防腐剂（苯甲酸、山梨酸）、咖啡因、可可碱和茶碱的离子色谱新方法。采用阴离子交换分析柱，40℃，45min，用含4%(v/v)乙腈的5mM NaH2PO4水溶液(pH 8.20)等度洗脱，用波长转换紫外吸光度检测器测定。所有分析物的检测限（信噪比 3:1）均低于亚杯/毫升水平。实验条件下，柠檬酸、苹果酸、酒石酸、抗坏血酸等多种有机酸均不干扰测定。该方法已成功应用于多种食品和药物制剂的分析，实际样品的平均回收率为85%至104%。通过该方法测定的所有分析物的水平与通过高效液相色谱程序获得的水平非常一致。结果还表明，离子色谱法可能是传统高效液相色谱法的有益替代品，用于分离和测定这些化合物。 (C) 2001 Elsevier Science B.V. 保留所有权利。
A novel ion chromatographic method was proposed for the simultaneous determination of artificial sweeteners (sodium saccharin, aspartame, acesulfame-K), preservatives (benzoic acid, sorbic acid), caffeine, theobromine and theophylline. The separation was performed on an anion-exchange analytical column operated at 40 degreesC within 45 min by an isocratic elution with 5 mM aqueous NaH2PO4 (pH 8.20) solution containing 4% (v/v) acetonitrile as eluent, and the determination by wavelength-switching ultraviolet absorbance detection. The detection limits (signal-to-noise ratio 3:1) for all analytes were below the sub-mug/ml level. Under the experimental conditions, several organic acids, including citric acid, malic acid, tartaric acid and ascorbic acid, did not interfere with the determination. The method has been successfully applied to the analysis of various food and pharmaceutical preparations, and the average recoveries for real samples ranged from 85 to 104%. The levels of all analytes determined by this method were in good agreement with those obtained by the high-performance liquid chromatographic procedure. The results also indicated that ion chromatography would be possibly a beneficial alternative to conventional high-performance liquid chromatography for the separation and determination of these compounds. (C) 2001 Elsevier Science B.V. All rights reserved.